LoVo人结肠癌细胞
产品介绍
目录号 Serial | TCHu 82 | 标识符 Identifier | CSTR:19375.09.3101HUMTCHu82 | 描述 Description | LoVo建自1971年,从诊断为结肠腺癌的56岁男性白人的一个转移到左锁骨上区的肿瘤结节建系。 癌基因C-myc, K-ras, H-ras, N-ras, Myb, sis 和fos的表达呈阳性。 癌基因N-myc的表达未做检测。 它在裸鼠中能成瘤。 与107细胞联合皮下接种5只裸鼠21天后全部成瘤。 表达肿瘤特异的核基质蛋白蛋白CC-3和CC-4。 在本库通过支原体检测。 在本库通过STR检测。 | 动物种别 Organism | 人 | 性别 Gender | 男 | 组织来源 Tissue and Cell Type | 直结肠腺癌,转移位置:左锁骨上区 | 形态 Morphology | 上皮细胞 | 培养基和添加剂 Complete Growth Medium and Culture Conditions | F-12K Nutrient Mixture (gibco by life technologies 21127-022),90%;优质胎牛血清,10%。 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。 | 供应限制 Permits and Restrictions | A。仅供研究之用。 | REFERENCE | Drewinko B , et al. Further biologic characteristics of a human carcinoembryonic antigen-producing colon carcinoma cell line. J. Natl. Cancer Inst. 61: 75-83, 1978. PubMed: 276641Drewinko B , Yand LY . Restriction of CEA synthesis to the stationary phase of growth of cultured human colon carcinoma cells. Exp. Cell Res. 101: 414-416, 1976. PubMed: 964319Drewinko B , et al. Establishment of a human carcinoembryonic antigen-producing colon adenocarcinoma cell line. Cancer Res. 36: 467-475, 1976. PubMed: 1260746Trainer DL , et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874Keesee SK , et al. Nuclear matrix proteins in human colon cancer. Proc. Natl. Acad. Sci. USA 91: 1913-1916, 1994. PubMed: 8127905Drewinko B , et al. Response of exponentially growing, stationary-phase, and synchronized cultured human colon carcinoma cells to treatment with nitrosourea derivatives. Cancer Res. 39: 2630-2636, 1979. PubMed: 445465Miranda L , et al. Isolation of the human PC6 gene encoding the putative host protease for HIV-1 gp160 processing in CD4+ T lymphocytes. Proc. Natl. Acad. Sci. USA 93: 7695-7700, 1996. PubMed: 8755538 |
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