MCF7 [MCF-7]人乳腺癌细胞(2013年新引进)
产品介绍
目录号
Serial | SCSP-531 | 标识符
Identifier | CSTR:19375.09.3101HUMSCSP531 | 描述
Description | 人乳腺癌细胞MCF7保留了分化乳腺上皮的许多特性。包括：能通过胞质雌激素受体加工雌二醇并能形成圆形复合物(domes)。该细胞含有WNT7B癌基因。肿瘤坏死因子α(TNF alpha)可以抑制MCF-7细胞生长。细胞经抗雌激素处理后能调节胰岛素样生长因子结合蛋白IGFBPS分泌。每个批次均通过本库支原体检测，结果为阴性。经本库STR检测结果无误。STR结果如下：D5S818: 11,12；D13S317: 11；D7S820: 8,9；D16S539: 11,12；vWA: 14,15；THO1: 6；Amelogenin: X；TPOX: 9,12；CSF1PO: 10 | 动物种别
Organism | 人；白人 | 性别
Gender | 女性，69岁 | 组织来源
Tissue and Cell Type | 乳腺、胸；分离自转移灶：胸腔积液 | 形态
Morphology | 上皮样，贴壁生长 | 培养基和添加剂
Complete Growth Medium and Culture Conditions | 人乳腺癌细胞MCF7培养液配方(100 ml)：
MEM (Invitrogen, 11090081)88 ml
FBS (Gibco)10 ml
Glutamax(invitrogen 35050061) 1 ml
Non-essential Amino Acids, 100X (Invitrogen, 11140050) 1 ml
Sodium Pyruvate 100 mM Solution (Invitrogen 11360070) 1 m
human recombinant insulin(SIGMA, 91077C-1G) 终浓度0.01 mg/ml
参考传代周期：5-7天
参考传代比例：1:3
参考换液频率：每周2-3次
冻存液配方：完全培养液95%，DMSO 5%
气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
注意事项：
a) 该细胞贴壁较慢，建议复苏、传代后让细胞贴壁48-72h后再进行后续实验操作。
b) 使用0.25%(w/v) Trypsin-0.53 mM EDTA消化细胞。
c) MCF7是一种生长缓慢的细胞系，它会以松散的三维团簇的形式出现，并伴有一些漂浮的活细胞。悬浮的活细胞可以通过离心后重新加入到培养瓶中培养。在复苏后前二代该细胞会出现贴壁缓慢的情况，是正常现象。松散的三维团簇细胞慢慢会扩散形成一个扁平的单层细胞。如果生长速度比正常情况慢，可以尝试另一批FBS(即血清批次的促生长能力可能不同)。此外，将血清浓度增加到20%也可能有助于改善生长。 | 供应限制
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