MDA-MB-231人乳腺癌细胞(2018年新引进)

产品介绍

目录号 Serial	SCSP-5043
标识符 Identifier	CSTR:19375.09.3101HUMSCSP5043
描述 Description	MDA-MB-231来自患有转移乳腺腺癌的51岁白种人女病人的胸水。在裸鼠和ALS处理的BALB/c小鼠中，它能形成低分化腺癌(III级)。细胞表达WNT7B致癌基因。此细胞株可能作为转染宿主。我库冻存时为P41代。每个批次均通过本库支原体检测，结果阴性。经本库STR检测无误，STR鉴定结果为：Amelogenin: X；CSF1PO: 12,13；D13S317: 13；D16S539: 12；D5S818: 12；D7S820: 8,9；THO1: 7,9.3；TPOX: 8,9；vWA: 15,18。
动物种别 Organism	人；白人
性别 Gender	女性，51岁
组织来源 Tissue and Cell Type	乳腺/乳房；来自转移灶：胸腔积液
形态 Morphology	上皮样，贴壁生长
培养基和添加剂 Complete Growth Medium and Culture Conditions	人乳腺癌细胞MDA-MB-231完全培养液配方(100 ml)： L-15 (Hyclone, SH30525.01 或Invitrogen 11415-064)90 ml FBS (Gibco) 10 ml 气相：空气，100%。温度：37摄氏度。参考传代比例：1：3 参考传代周期：4-6天参考换液频率：每周2次冻存液配方：完全培养液95%，DMSO 5% 【注意事项：】 1.该细胞使用的L-15基础培养液由磷酸盐和游离碱氨基酸代替了碳酸氢钠缓冲体系。该培养基必须在没有CO2平衡的环境中培养细胞。不能在普通的5% CO2细胞培养箱中培养，培养液颜色会明显变黄。 2.细胞形态大部分为纺锤状，也有部分细胞呈圆形，存在少量悬浮细胞。 3.如果发现细胞生长极其缓慢，可提高血清含量至20%。 4. 我库冻存时，每支冻存管约含7×105细胞量，体积为500μl，预期存活率70%，建议复苏至1个T25培养瓶中。
供应限制 Permits and Restrictions	A。仅供研究之用。
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