LoVo人结直肠癌细胞(白人)

产品介绍

目录号 Serial	SCSP-514
标识符 Identifier	CSTR:19375.09.3101HUMSCSP514
描述 Description	人结直肠癌细胞LoVo构建于1971年，来源于一名56岁的白人男性结直肠癌患者转移到左锁骨位置的一个肿瘤结节。CSAp和结肠抗原3(colon antigen 3)表达阴性。肿瘤基因c-myc、K-ras、H-ras、 N-ras、Myb, sis和fos表达阳性，但未检测到 N-myc表达。该细胞表达肿瘤特定的核基质蛋白CC-3和CC-4。每个批次均通过本库支原体检测，结果阴性。经本库STR检测结果无误，STR结果如下：D5S818: 11,13；D13S317: 8,11；D7S820:9.3,10,11；D16S539: 9,12；vWA: 17,18；THO1: 9.3；Amelogenin: X,Y；TPOX: 8,9；CSF1PO: 10,11,13,14
动物种别 Organism	人；白人
性别 Gender	男性，56岁
组织来源 Tissue and Cell Type	结肠；分离自转移灶：左锁骨上区域
形态 Morphology	上皮样，贴壁生长
培养基和添加剂 Complete Growth Medium and Culture Conditions	完全培养液配方(100 ml) Ham's F-12K (Kaighn's) Medium (Invitrogen, 21127-022) 90 ml FBS (Gibco) 10 ml 参考传代周期：2-3天参考传代比例：1:3-1:5 参考换液频率：每周2-3次冻存液配方：完全培养液95%，DMSO 5% 气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
供应限制 Permits and Restrictions	A。仅供研究之用。
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