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Anti-Rsk1 Antibody

品牌
Sigma-Aldrich
货号
07-1477
规格纯度
from rabbit, purified by affinity chromatography
参考价格
5520.38 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Rsk1, Rsk2, and Rsk3 (ribosomal protein S6 kinase, 90kDa, polypeptide 1,2,3; S6K-alpha 1,2,3) are Ser/Thr kinases which are activated by MAP Kinases, and are alternatively referred to as MAPKAP kinases 1a, 1b, and 1c. Following activation, Rsk isoforms translocate to the nucleus, where Rsk2 phosphorylates Ser10 of Histone H3, a critical step in the stimulation of immediate-early gene expression. Rsk1 is able to phosphorylate Ser133 of CREB, activating it as a transcription factor. Rsk1 activation can be monitored by phosphorylation state specific antibodies directed against sites involved in its activation.

特异性

Recognizes p90 RSK1.

免疫原

Synthetic peptide corresponding to the c-terminal sequence of human p90 RSK1 (C-LAQRRVRKLPSTTL).

应用

Immunoprecipitation: 4 µg of a previous lot of this antibody was used per 1500 µg of crude protein sample in Immunoprecipitation.

ELISA: Recommended.
Optimal working dilutions must be determined by end user.

Western Blot: A previous lot of this antibody was tested on multiple lysates. See datasheet.
Anti-Rsk1 Antibody is an antibody against Rsk1 for use in WB, IP, ELISA.

质量

Routinely evaluated by Western Blot on NIH/3T3 lysates.

Western Blot Analysis:
1:500-1:1,000 dilution of this lot detected RSK1 on 10 μg of NIH/3T3 lysates.

目标描述

83 kDa

联系

Replaces: 04-417

外形

Affinity purified
Purified rabbit polyclonal IgG in buffer containing TBS with 0.01% Sodium Azide in 50% glycerol.

储存及稳定性

Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆polyclonal
纯化方式affinity chromatography
species reactivityrat, mouse, human
technique(s)ELISA: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型IgG
NCBI登记号NM_001006665.1
UniProt登记号Q15418
运输dry ice
Gene Informationhuman ... RPS6KA1(6195)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1

Sigma-Aldrich

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