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Anti-phospho-Akt (Thr308) Antibody

品牌
Sigma-Aldrich
货号
07-1398
包装型号
规格纯度
from rabbit
参考价格
5016.79 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Akt/PKB is a Ser/Thr kinase and a major known effecter of the PI3 Kinase pathway. It is involved in multiple signaling pathways that relate to many biological processes including metabolism, apoptosis, cell cycle control, angiogenesis, differentiation, and cell growth and proliferation. In mammals, three isoforms of Akt (Akt1/PKCα, Akt2/PKBβ, and Akt3/PKBγ) exists. They exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt1 is the predominant isoform that is in most tissues and is thought to have a dominant role in growth, survival, embryonic development, and adipocyte differentiation. Akt2 is correlated with the regulation of glucose homeostasis and is the predominant isoform expressed in insulin-responsive tissues. Akt3 is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal Pleckstrin Homology (PH) domain that provides a lipid-binding module, a central catalytic domain containing Thr308, and a C-terminal hydrophobic motif containing Ser473. The activation of AKT is dependent on a dual regulatory mechanism that requires both its translocation to the plasma membrane and dual phosphorylation on Thr308 and Ser473 by PDK1 and the TORC2 complex, respectively.

免疫原

Epitope: Thr308 (Catalytic Domain)
KLH-conjugated synthetic peptide containing the Thr308 sequence and surrounding amino acids of human, mouse, rat, bovine and chicken Akt1. Human and mouse Akt2 shares 11/12 amino acids and rat Akt3 shares 10/12 amino acids with the peptide immunogen sequence.

应用

Immunohistochemistry only

Western Blot Analysis:
Not recommended for WB as it gives multiple bands. Please refer to 05-802R

This Anti-phospho-Akt (Thr308) Antibody is validated for use in IH(P) for the detection of phospho-Akt (Thr308).

质量

Immunohistochemistry Analysis:
Antibody diluted to 1:500, IHC-Select Detection with HRP-DAB.

目标描述

60 kDa

外形

Antigen affinity purified against both the phosphorylated and the non-phosphorylated peptides of Akt (Thr308).

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
纯化方式affinity chromatography
species reactivitycanine, mouse
species reactivity (predicted by homology)rat (based on 100% sequence homology), dog (based on 100% sequence homology), bovine (based on 100% sequence homology), human (based on 100% sequence homology), Xenopus (based on 100% sequence homology), chicken (based on 100% sequence homology)
technique(s)immunohistochemistry: suitable (paraffin)
同位素/亚型IgG
适用性not suitable for Western blot
NCBI登记号NP_001014431
UniProt登记号P31749
运输wet ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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