产品说明
一般描述
NR2 or glutamate receptor, ionotropic, N-methyl D-aspartate 2 (NMDA2; NMDAR2) along with the NR1 subunit forms a heterodimer that make up the NMDA receptor. By alternative splicing there are various isoforms of NR2 classified as NR2A through D. The NMDA receptor (NMDAR) is characterized as a ionotropic receptor for glutamate. As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor. A distinct characteristic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium. The NR1 subunit binds glycine while the NR2 subunit binds glutamine for activation. Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations. The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell. Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory.
特异性
Recognizes NR2A, MW ~170 kDa.
免疫原
6His-tagged fusion protein corresponding to amino acids 1265-1464 of mouse NMDA receptor 2A (NR2A).
应用
Detect NR2A using this Anti-NR2A Antibody validated for use in IP & WB.
Immunoprecipitation:
4 µg of a previous lot immuno-precipitated NR2A from rat brain hippocampus membranes and rat brain microsomal preparations.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
质量
Evaluated by western blot in rat brain microsomal preparations (Catalog # 12-144).
Western Blot Analysis:
0.5-2 µg/mL of this antibody detected NR2A in rat brain microsomal preparations (Catalog # 12-144).
目标描述
~170 kDa
外形
Format: Purified
Protein A purified
Purified rabbit polyclonal IgG in buffer containing 70% storage buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol.
储存及稳定性
Stable for 1 year at -20ºC from date of receipt.
分析说明
Control
Brain tissue.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | polyclonal |
species reactivity | rat, mouse |
manufacturer/tradename | Upstate® |
technique(s) | immunoprecipitation (IP): suitable western blot: suitable |
同位素/亚型 | IgG |
NCBI登记号 | NM_008170 |
UniProt登记号 | Q12879 |
运输 | wet ice |
Gene Information | human ... GRIN2A(2903) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |