产品说明
一般描述
Tumor susceptibility 101 (TSG101) is encoded by the gene mapped to human chromosome 11p15.1–15.2. The encoded protein is a member of catalytically inactive ubiquitin E2 variant (UEV) family. Tsg101 is characterized with an ubiquitin E2 variant (UEV) domain in its N-terminal end involved in HIV-1 budding and the vacuolar protein sorting (VPS) pathway.
免疫原
syntheitic peptide corresponding to amino acids 254-272 located near the C-terminus of human TSG101, conjugated to KLH. This sequence is identical in rat and mouse TSG101.
应用
Anti-TSG101 antibody produced in rabbit has been used in western blot analysis.
生化/生理作用
Tumor susceptibility 101 (TSG101) is implicated in various cellular function such as, ubiquitination, transcriptional regulation, endosomal trafficking and cell proliferation. Mutation in the gene leads to various human cancers.Tsg101 plays a crucial role in cell growth, cell survival, and normal function of embryonic and adult tissues. TSG101 facilitates infectious human immunodeficiency virus (HIV-1) budding by linking the p6 late domain to vacuolar protein sorting machinery. Tsg101 is a vital constituent of the endosomal sorting complex required for transport (ESCRT) machinery, that helps in synthesis of multi-vesicular bodies, mainly the formation and scission of the intraluminal vesicles, and is thus, crucial for the degradation and recycling of receptors localized on plasma membrane. Tsg101 expression is controlled by an intrinsic autoregulatory mechanism and by ubiquitination catalyzed by various ubiquitin ligases.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium axide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD08705528 |
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 偶联物 | unconjugated |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen 46 kDa |
| species reactivity | rat, human, mouse |
| 浓度 | ~2 mg/mL |
| technique(s) | western blot: 0.5-1 μg/mL using cell lysates of rat fibroblast Rat-1 and mouse fibroblast NIH3T3 cells western blot: 2-4 μg/mL using cell lysates of human A431 cells |
| UniProt登记号 | Q99816 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... TSG101(7251) mouse ... Tsg101(22088) rat ... Tsg101(292925) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
