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Anti-Cortactin (KE-20) antibody produced in rabbit

品牌
Sigma-Aldrich
货号
C7112
规格纯度
IgG fraction of antiserum, buffered aqueous solution
参考价格
4731.57 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Cortactin is encoded by the CTTN gene on chromosome 11 in humans. It is expressed in most adherent cells and is a prominent substrate of protein-tyrosine kinase Src. The protein localizes within lamellipodia and at the cell surface. Cortactin is also called as EMS1.
Cortactin is an F-actin binding protein. It belongs to a highly conserved family of perimembrane signaling protein. Cortactin contains a unique multi-domain structure consisting of 6.5 tandem repeats of a 37-amino acid sequence required for direct binding to F-actin, followed by an α-helix region, a proline-rich region, and three tyrosine residues targeted by Src related kinases. The C-terminal region contains a Src-homology 3 (SH3) domain.

免疫原

synthetic peptide corresponding to the mid-region of human cortactin (amino acids 361-380) conjugated to KLH.

应用

Anti-Cortactin (KE-20) antibody produced in rabbit is suitable for indirect immunofluorescence at a dilution of 1:500 using mouse fibroblasts NIH3T3 cell line and western blotting at a dilution of 1:1,000 using cytosol extract of rat brain, whole extract of chicken embryo fibroblasts, or whole cell extract of the human epidermoid carcinoma A431 cell line.

生化/生理作用

Cortactin is a cytoskeleton protein that facilitates the assembly of cortical actin. It is activated by phosphorylation of tyrosine kinases or serine/threonine kinases. It plays an important role in promoting lamellipodia formation, invadopodia formation, cell migration, and endocytosis. Cortactin regulates the interactions between components of adherens-type junctions and helps in organizing the cytoskeleton, cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. It acts as a regulator in invasion and migration of tumor cells. Overexpression of cortactin causes osteosarcoma carcinogenesis and along with FAK increases the risk of oral premalignant lesions.

外形

Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD03791487
NACRESNA.41

产品性质

生物来源rabbit
质量水平200
偶联物unconjugated
抗体形式IgG fraction of antiserum
antibody product typeprimary antibodies
克隆polyclonal
形式buffered aqueous solution
species reactivitymouse, human, rat, chicken
technique(s)indirect immunofluorescence: 1:500 using mouse fibroblasts NIH3T3 cell line
western blot: 1:1,000 using cytosol extract of rat brain, whole extract of chicken embryo fibroblasts, or whole cell extract of the human epidermoid carcinoma A431 cell line
UniProt登记号Q14247
运输dry ice
储存温度−20℃
Gene Informationhuman ... CTTN(2017)
mouse ... Cttn(13043)
rat ... Cttn(60465)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKnwg
闪点(F)Not applicable
闪点(C)Not applicable

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