产品说明
一般描述
Pyruvate Kinase (PK) is a key regulatory enzyme in glycolysis and has four known isoforms, namely, L, R, M1 and M2. The M2 isoform has been linked to cancer metastasis. PK isoform M1 is expressed during the development of the embryo and is the dominant isoform in cardiac, brain and skeletal muscle tissues.
特异性
Anti-PKM2 (isoform M1) antibody is specific for human, mouse and rat PKM2 (Isoform M1)/PKM1. In immunoblotting, detection of the PKM2 (Isoform M1) /PKM1 band is specifically inhibited by the immunizing peptide.
应用
Anti-PKM2 (isoform M1) antibody produced in rabbit has been used in:
- immunoblotting
- immunoprecipitation
- immunohistochemistry
- immunocytochemistry
生化/生理作用
Pyruvate kinase (PK) is a key enzyme in the glycolytic pathway. Knockdown of the M2 isoform in human cancer cell lines and its replacement by the M1 isoform has been shown to lead to reversal of the Warburg effect, and reduced ability to form tumors in mouse xenografts. Phosphorylation of the M2 isoform at Tyr105 inhibits its activity and is common in human cancers, suggesting that Tyr105 is a critical metabolic switch in cancer cells that promotes tumorigenesis.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
产品性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 偶联物 | unconjugated |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~60 kDa |
| species reactivity | human, rat, mouse |
| 增强验证 | recombinant expression Learn more about Antibody Enhanced Validation |
| 浓度 | ~1.5 mg/mL |
| technique(s) | immunocytochemistry: 5-10 μg/mL using HeLa cells immunohistochemistry: 20-30 μg/mL using formalin-fixed paraffin embedded human colon immunoprecipitation (IP): 2-4 μg using rat brain extract (S2 fraction) western blot: 1-2 μg/mL using mouse brain extract (S2 fraction) |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... PKM2(5315) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
