产品说明
一般描述
Dishevelled 3 (DVL3) is a phosphoprotein rich in serine and threonine. DVL3 gene is mapped to human chromosome 3q27.1 and the encoded protein is widely expressed in fetal tissues and specifically expressed in various adult tissues.
特异性
Anti-DVL3 (C-terminal region) specifically recognizes human DVL3.
应用
Anti-DVL3 (C-terminal region) antibody produced in rabbit has been used in:
- immunohistochemistry
- chromatin immunoprecipitation
- western blotting
- immunofluorescence
- liquid chromatography/mass spectrometry (LC-MS/MS)
生化/生理作用
Dishevelled 3 (DVL3) participates in the wingless-related integration site (Wnt) signaling pathway. It plays a key role in hippocampal neurogenesis regulation. DVL3, like other members of the DVL family, functions upstream of glycogen synthase kinase-3 (GSK3) to modulate the canonical wnt pathway. Knockdown of DVL3 has been shown to attenuate the activation of the wnt signaling pathway by Wnt3A. DVL3 functions in redundant pathways with DVL1 and DVL2, and is necessary in normal cardiac, cochlea, and neural tube development in mice. A frame shift mutation in DVL3 is implicated in Robinow syndrome. Allelic variation in the DVL3 gene may be associated with major depressive disorder.
目标描述
DVL3 (C-terminal region) is a member of a multi-gene family which shares strong similarity with the Drosophila dishevelled gene, dsh. The Drosophila dishevelled gene encodes a cytoplasmic phosphoprotein that regulates cell proliferation.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
储存及稳定性
Store at –20 ℃. For continuous use, the product may be stored at 2–8 ℃ for up to one month. For extended storage, freeze in working aliquots at –20 ℃. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 偶联物 | unconjugated |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~95 kDa |
| species reactivity | human |
| 包装 | antibody small pack of 25 μL |
| 增强验证 | recombinant expression Learn more about Antibody Enhanced Validation |
| 浓度 | ~1.5 mg/mL |
| technique(s) | immunoprecipitation (IP): 1.5-3 μg using HEK-293T cell lysates overexpressing human DVL3 western blot: 0.025-0.05 μg/mL using HEK-293T cell lysates overexpressing human DVL3 |
| UniProt登记号 | Q92997 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... DVL3(1857) mouse ... Dvl3(13544) rat ... Dvl3(303811) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
