产品说明
一般描述
Tensin 1 (TNS1) is encoded by the gene mapped to human chromosome 2q35. The encoded protein is localized to focal adhesions and is mainly expressed in renal tubular epithelial cells (TECs).
免疫原
synthetic peptide corresponding to a sequence located in the internal region of human tensin1, conjugated to KLH. The corresponding sequence is identical in rat and mouse tensin1.
应用
Anti-Tensin 1 antibody may be used in immunofluorescence and immunohistochemistry.
Anti-Tensin 1 antibody produced in rabbit has been used in immunostaining and immunoblotting.
生化/生理作用
Tensin 1 associates with actin, integrin, FAK, p130Cas, DLC-1 and protein phosphatase PP1 which affects cell polarization, migration and invasion. Tensin 1 undergoes extensive Ser/Thr and Tyr phosphorylation induced by extracellular matrix (ECM), various growth factors and oncogenes. In addition, tensin 1 is cleaved by calpain II, a focal adhesion protease, suggesting that tensin 1 plays important roles in organizing the actin cytoskeleton and mediating signal transduction.
Tensin 1 (TNS1) contributes to transforming growth factor (TGF)-β-induced myofibroblast differentiation and extracellular fibronectin and collagen matrix formation. Disruption in the gene and associated signaling might hinder tissue fibrosis. The encoded protein anchors to stress fibers by interacting with cytoplasmic tails of integrins and increases metastatic abilities of cancer cells. Tensin plays a vital role in activation of the c-Jun N-terminal kinases (JNK) and p38 pathways.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 偶联物 | unconjugated |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen 225 kDa (rat) antigen ~150 kDa (human) |
| species reactivity | rat, chicken, human |
| 增强验证 | recombinant expression Learn more about Antibody Enhanced Validation |
| 浓度 | ~1.0 mg/mL |
| technique(s) | immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin-embedded rat heart. indirect immunofluorescence: 3-6 μg/mL using chicken embryo fibroblast cells. western blot: 1-2 μg/mL using lysates of HEK-293T cells overexpressing human tensin 1 |
| UniProt登记号 | Q9HBL0 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... TNS1(7145) mouse ... Tns1(21961) rat ... Tns1(301509) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
