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Anti-Histone Macro H2A.1 Antibody

品牌
Sigma-Aldrich
货号
ABE215
规格纯度
from rabbit, purified by affinity chromatography
参考价格
6694.05 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Histone MacroH2A (mH2A) is a variant histone also known as H2AFY. mH2A is enriched in chromatin of the female inactive X chromosome and in highly methylated CpG loci. This histone variant is known to repress RNA polymerase II-driven transcription at the level of transcriptional initiation. In contrast to most histone variants containing only subtle changes, mH2A contains a large non-histone domain (the macro domain) on its C-terminus, resulting in a histone approximately three times the size of conventional H2A. There are 3 isoforms of mH2A, two of which (mH2A1.1, mH2A1.2 ) are formed by alternative splicing and another (mH2A2) from a separate gene. There are two phosphorylation sites within the basic hinge region of mH2A: Thr128 and Ser137. Phospho-Ser137 is enriched in mitosis, implicating mH2A in the regulation of posttranslational modifications throughout the cell cycle.

特异性

This antibody recognizes Histone MacroH2A.1 at the macro domain.

免疫原

GST-tagged recombinant protein corresponding to mcro domain of mouse Histone MacroH2A.1.
Epitope: Macro domain

应用

Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone MacroH2A.1 in NIH/3T3, A431, and HeLa cells.
Anti-Histone Macro H2A.1 Antibody is a Rabbit Polyclonal Antibody for detection of Histone Macro H2A.1 also known as H2A histone family member Y, histone macroH2A1.2 & has been validated in WB, ICC.

质量

Evaluated by Western Blot in HeLa acid extract.

Western Blot Analysis: 0.2 µg/mL of this antibody detected histone MacroH2A.1 on 10 µg of HeLa acid extract.

目标描述

~ 40 kDa observed

联系

Replaces: 07-219

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
HeLa acid extract

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
纯化方式affinity chromatography
species reactivitymouse, human
technique(s)immunocytochemistry: suitable
western blot: suitable
NCBI登记号NP_001152985.1
UniProt登记号Q9QZQ8
运输wet ice
Gene Informationhuman ... H2AFJ(55766)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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