Anti-DNMT1 antibody produced in rabbit

产品说明

一般描述

In humans, the gene encodes a DNA (cytosine-5)-methyltransferase with 1616 amino acids. The N-terminal contains the regulatory domain, while the C-terminal region contains the catalytic domain. Two isoforms of DNMT1 have been isolated, DNMT1a and DNMT1b, the difference residing in 16 extra amino acids within the latter.
Human DNA methyltransferase 1 (Dnmt1) is a 1616 amino acids protein; the N-terminal two-thirds of the protein is considered to be the regulatory domain, while the C-terminal region contains the catalytic domain.

免疫原

synthetic peptide corresponding to amino acids 1581-1595 of rat DNMT1, conjugated to KLH via an N-terminal added lysine residue. The sequence is conserved in human and mouse.

应用

Anti-DNMT1 antibody produced in rabbit has been used in western blot analysis, and staining for confocal laser scanning microscopy.

生化/生理作用

DNA methyltransferase 1 (Dnmt1) is part of different complexes; it can establish a repressive transcription complex consisting of the histone deacetylase 4 (HDAC4), DNA methyltransferase 1-associated Protein (DMAP1) and DNMT1 itself. Consistent with the role of DNA methylation in gene silencing, DNMT1 can associate with HDAC1 and MECP2 (methyl CpG binding protein 2).
DNMT1 protein is important for the maintenance of methylation, as well as for the novo methylation activities occurring in somatic cells of vertebrates. It establishes a repressive transcription complex at replication foci with histone deacetylase HDAAC4 and DMAP1.

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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