产品说明
一般描述
Y-box-binding protein (YB-1) also known as nuclease-sensitive element-binding protein 1 (NSEP1) is a member of the Cold-Sock Domain (CSD) containing proteins. It is composed of three domains: the N-terminal which is thought to function as a trans-activation regulatory domain, a highly conserved nucleic acid binding domain (the CSD), and C-terminal domain, involved in protein-protein interactions.
特异性
Anti-YB-1 (C-terminal) antibody is specific for YB-1 in rats, mice and humans. The immunizing peptide specifically inhibits the detection of the YB-1 band by immunoblotting.
免疫原
synthetic peptide corresponding to amino acids 307-324 of human YB-1, conjugated to KLH. The corresponding sequence is identical in rat and mouse YB-1 proteins.
应用
Anti-YB-1 (C-terminal) antibody has been used in
- immunoblotting
- immunoprecipitation
- immunofluorescence
- double-immunostaining
生化/生理作用
Y-box-binding protein (YB-1) is shown to bind to the Y-box promoter element to regulate gene expression. It plays an important role in a wide variety of cellular functions such as transcriptional and translation regulation, DNA repair, drug resistance, and stress responses.
外形
Solution in 0.01 M phophate buffered saline, pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 偶联物 | unconjugated |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~50 kDa |
| species reactivity | rat, mouse, human |
| 浓度 | ~1.0 mg/mL |
| technique(s) | immunoprecipitation (IP): 5-10 μg using HEK-293T cell lysates indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde-fixed NIH-3T3 cells western blot: 0.5-1 μg/mL using PC12 cell lysates |
| UniProt登记号 | P67809 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... YBX1(4904) mouse ... Ybx1(22608) rat ... Ybx1(500538) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
