Anti-monomethyl Histone H3 (Lys4) Antibody

产品说明

一般描述

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). Histone H3 is acetylated on Lys14 by Gcn5, and histone H3 phosphorylation at Ser10 facilitates acetylation of Lys14 by Gcn5 in vitro. Moreover, Lys14 is involved in chromatin remodeling, and RSC4 (part of the RSC chromatin remodeling complex) contains essential tandem bromodomains that require H3 Lys14 acetylation for gene activation.

免疫原

KLH-conjugated linear peptide corresponding to the N-terminus of human monomethyl Histone H3 (Lys4).
Epitope: N-terminus

应用

Anti-monomethyl Histone H3 (Lys4) is an antibody against monomethyl Histone H3 (Lys4) for use in WB, IP, IH, Dot Blot.
Research Sub Category
Nuclear Receptors
Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected monomethyl Histone H3 (Lys4) in an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated monomethyl Histone H3 (Lys4) in 500 µg of HeLa acid extract.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected monomethyl Histone H3 (Lys4) in human and rat testis tissue.
Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected monomethyl Histone H3 (Lys4) in 10 µg of HeLa acid extract.

目标描述

~17 kDa observed

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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