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Anti-Human IgA (α-chain specific)-Alkaline Phosphatase antibody produced in goat

品牌
Sigma-Aldrich
货号
A9669
规格纯度
affinity isolated antibody, buffered aqueous solution
参考价格
5355.51 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Immunoglobulin A (IgA) is the most abundant antibody isotype produced in humans. IgAs are heterogeneous and exist as two IgA subtypes. It has the ability to form multimers by interacting with the joining (J) chain.It is present in large quantities in blood. IgA antibodies protect mucosal surfaces against invading pathogens.
Specificity of the Alkaline Phosphatase Conjugated Anti-Human IgA is determined by Enzyme Linked Immunosorbent Assay (ELISA). Identity and purity of the antibody is established by immunoelectrophoresis (IEP) prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion versus anti-goat IgG and anti-goat whole serum results in single arcs of precipitation.

特异性

The conjugate is specific for human IgG. No cross-reaction with Mouse and Rat IgG.

免疫原

Purified human IgA

应用

Human milk samples were analyzed for the presence of IgA antibodies specific to certain antigens of H. pylori by western blot using alkaline-phosphate-conjugated goat anti-human IgA antibody.
Anti-Human IgA (α-chain specific) Alkaline Phosphatase antibody produced in goat has been used in quantitative sandwich enzyme-linked immunosorbent assay (ELISA) and immunoassays.
The product may also be used for dot blot (1:30,000) and immunohistochemistry (at 1:100 dilutions, using formalin-fixed, paraffin-embedded sections).
Milk samples from 10 mothers were probed for bound milk IgA by western blot using alkaline-phosphatase goat anti-human IgA antibody.Elisa Assays were performed using alkaline phosphatase conjugated gaot anti-human IgA chain specific antibody (1:1000) to detect IgA immunoglobulins in serum and seminal plasma samples from infertile men.

生化/生理作用

Immunoglobulin A (IgA) antibody is a secretory antibody present abundantly in mucous linings of gastrointestinal, respiratory and genitourinary tracts, tears and saliva. Thus, secretory IgA contributes to the humoral defense mechanism against the pathogens on mucosal surfaces.
IgA is a secretory glycoprotein antibody that regulates the physiological balance between commensal intestinal bacteria and the defenses of the host immune system. It is the highest generated antibody isotype. IgAs are mainly secreted in the intestinal mucosa and prevent the commensal microorganisms from invading the intestines

外形

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide

储存及稳定性

Store at 0-5℃. Do Not Freeze.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD00162413
NACRESNA.46

产品性质

质量水平200
生物来源goat
偶联物alkaline phosphatase conjugate
抗体形式affinity isolated antibody
antibody product typesecondary antibodies
克隆polyclonal
形式buffered aqueous solution
technique(s)direct ELISA: 1:35,000
dot blot: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
运输wet ice
储存温度2-8℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

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