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BES, Free Acid, ULTROL® Grade

品牌
Millipore
CAS
10191-18-1
货号
391334
规格纯度
BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable.
参考价格
969.83 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable (Absorbance: <0.05 at 260 nm, 100 mM). Chen and Okayama describe an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The slow formation appears to be the most critical factor contributing to the high efficiency of stable transformations. Has a pKa of 7.12 at 20°C.
Absorbance (100 mM, 260 nm):≤0.05.
BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable. Chen and Okayama described an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The gradual formation of the calcium phosphate-DNA complex and its slow precipitation onto cells appears to be the most critical factor contributing to the high efficiency of stable transformations.

包装

25 g in Plastic ampoule
100 g in Glass bottle

警告

Toxicity: Standard Handling (A)

重悬

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 2 weeks at 4°C.

其他说明

De Graeve, K.G., et al. 1994. J. Appl. Bacteriol. 76, 55.
Chen, C.A., and Okayama, H. 1988. Biotechniques6, 632.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTROL is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

经验(实验)分子式C6H15NO5S
分子量213.25
MDL编号MFCD00007533

产品性质

质量水平100
测定≥99% dry basis (titration)
形式solid
manufacturer/tradenameCalbiochem®
储存条件OK to freeze
颜色 white
pKa (20 ℃)7.12
溶解性water: 1 mg/mL
痕量阳离子heavy metals: ≤1 ppm
运输ambient
储存温度15-25℃
InChI1S/C6H15NO5S/c8-4-1-7(2-5-9)3-6-13(10,11)12/h8-9H,1-6H2,(H,10,11,12)
InChI keyAJTVSSFTXWNIRG-UHFFFAOYSA-N

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

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