产品说明
一般描述
BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable (Absorbance: <0.05 at 260 nm, 100 mM). Chen and Okayama describe an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The slow formation appears to be the most critical factor contributing to the high efficiency of stable transformations. Has a pKa of 7.12 at 20°C.
Absorbance (100 mM, 260 nm):≤0.05.
BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable. Chen and Okayama described an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The gradual formation of the calcium phosphate-DNA complex and its slow precipitation onto cells appears to be the most critical factor contributing to the high efficiency of stable transformations.
包装
25 g in Plastic ampoule
100 g in Glass bottle
警告
Toxicity: Standard Handling (A)
重悬
Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 2 weeks at 4°C.
其他说明
De Graeve, K.G., et al. 1994. J. Appl. Bacteriol. 76, 55.
Chen, C.A., and Okayama, H. 1988. Biotechniques6, 632.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTROL is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| 经验(实验)分子式 | C6H15NO5S |
| 分子量 | 213.25 |
| MDL编号 | MFCD00007533 |
产品性质
| 质量水平 | 100 |
| 测定 | ≥99% dry basis (titration) |
| 形式 | solid |
| manufacturer/tradename | Calbiochem® |
| 储存条件 | OK to freeze |
| 颜色 | white |
| pKa (20 ℃) | 7.12 |
| 溶解性 | water: 1 mg/mL |
| 痕量阳离子 | heavy metals: ≤1 ppm |
| 运输 | ambient |
| 储存温度 | 15-25℃ |
| InChI | 1S/C6H15NO5S/c8-4-1-7(2-5-9)3-6-13(10,11)12/h8-9H,1-6H2,(H,10,11,12) |
| InChI key | AJTVSSFTXWNIRG-UHFFFAOYSA-N |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
