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Anti-Mouse IgG (Fc specific)–Alkaline Phosphatase antibody produced in goat

抗 小鼠 IgG(Fc 特异性)-碱性磷酸酶 山羊抗

品牌
Sigma-Aldrich
货号
A1418
规格纯度
affinity isolated antibody, buffered aqueous solution
参考价格
1578.27 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

IgG antibody plays a crucial role in humoral immune responses such as complement activation, phagocytosis, placental transport and cell surface-receptor binding. Anti-mouse IgG (Fc specific)–alkaline phosphatase antibody (diluted 1: 10,000 in PBS-Tween) can be used in ELISA. It is also useful in immunoblotting. Goat anti-mouse IgG (Fc specific)–alkaline phosphatase antibody reacts specifically with mouse IgG Fc fragment, IgG and its subclasses IgG1, IgG2a, IgG2b and IgG3 but not with human IgG, and mouse IgA or IgM.

免疫原

Purified mouse IgG Fc fragment.

应用

Anti-mouse IgG (Fc specific)–alkaline phosphatase antibody can be used in ELISPOT (immunospot) assay. It can also be used in immunohistochemistry and western blot.
Alkaline phosphatase-conjugated goat anti-mouse Fc specific antibody was used as a secondary antibody in ELISA assays at a dilution of 1:1000 in PBS/0.1% Tween and 1% BSA for 1.5 hours at 37℃. Antibody was developed using 4-nitrophenyl phosphate (Sigma) as a substrate for 30 minutes at 37℃.

其他说明

Antibody adsorbed with human IgG.

外形

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide

制备说明

Adsorbed to reduce background with human samples.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD00162626
NACRESNA.46

产品性质

质量水平200
生物来源goat
重组expressed in goat
偶联物alkaline phosphatase conjugate
抗体形式affinity isolated antibody
antibody product typesecondary antibodies
克隆polyclonal
形式buffered aqueous solution
species reactivitymouse
should not react with human
technique(s)direct ELISA: 1:40,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25
western blot: 1:50,000-1:100,000 using total cell extract of HeLa cells
运输wet ice
储存温度2-8℃

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

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