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β-Galactosidase from Escherichia coli

β 半乳糖苷酶 来源于大肠杆菌

品牌
Sigma-Aldrich
CAS
9031-11-2
货号
48275
规格纯度
suitable for enzyme immunoassay, lyophilized, powder, ~140 U/mg
参考价格
2843.4 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

应用

Aqueous solution may be used directly for derivatization without previous dialysis or gel permeation chromatography.

生化/生理作用

β-半乳糖苷酶可将乳糖切割成单糖成分(葡萄糖和半乳糖)。它还在反馈环中催化葡萄糖转糖基化为β-半乳糖苷酶的诱导物 - 异乳糖。

包装

Bottomless glass bottle. Contents are inside inserted fused cone.

物理属性

四聚体的分子量为465 kDa(每个亚基116.3 kDa)

单位定义

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 2-nitrophenyl-β-D-galactopyranoside (Cat. No. 73660) per minute at pH 7.8 and 37 ℃

其他说明

Used in a highly sensitive enzyme-linked immunoassay of human IgG with 4-methylumbelliferyl-β-D-galactoside (Catalog No. 69590); Evaluation of β-D-galactosidase (E. coli) and horse radish peroxidase as labels by sandwich enzyme immunoassay technique; Enzyme immunoassay of thyrptropin releasing hormone (TRH); Preparation of enzyme-antibody conjugates

基本信息

酶学委员会(EC)编号3.2.1.23 ( BRENDA | IUBMB )
EC 号232-864-1
MDL编号MFCD00130623
NACRESNA.54

产品性质

形式powder
质量水平100
质量lyophilized
specific activity~140 U/mg
分子量Mr ~135000
包含 phosphate buffer as stabilizer
sucrose as stabilizer
technique(s)enzyme immunoassay: suitable
运输wet ice
储存温度−20℃

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, type N95 (US)

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