位置:首页 > 品牌 > Sigma-Aldrich >

Creatinase from microorganisms

肌酸脱水酶 来源于放线杆菌

品牌
Sigma-Aldrich
CAS
37340-58-2
货号
C2409
规格纯度
lyophilized powder, ≥4 units/mg solid
参考价格
5043.21 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Creatinase is a homodimer that catalyzes hydrolysis of creatine. It consists of two monomer subunit and two defined domains; N and C terminal domains. C terminal fold have both the α helices and anti-parallel β sheet within two structurally similar domains. In between these two domains, there is a sulfhydryl group that acts as active site and the activity is metal independent.

应用

Creatinase mixed with sarcosine oxidase may be used to determine the level of creatine in different pH, temperature, enzyme ratio, and buffer concentration. It may also be used to determine the plasma creatinine level by using a centrifugal analyser.

包装

1000 units in poly bottle

生化/生理作用

Creatinase accelerates the conversion reaction of creatine and water molecule to sarcosine and urea. It always acts in homodimer state and is induced by choline chloride.

物理属性

Isoelectric point: 4.6 ± 0.1
Michaelis constant: 1.9 x 10‾2M (Creatine)
Structure: 2 subunits per mole of enzyme
Inhibitors: Cu++, Hg++, Ag+
Optimum pH: 8.0
Optimum temp: 40℃
pH Stability: pH 5.5 − 9.0 (25℃, 16hr)
Thermal stability: Below 50℃ (pH 7.5, 30 min)

单位定义

One unit will hydrolyze 1.0 μmole of creatine to urea and sarcosine per min at pH 7.5 at 37 ℃.

外形

Lyophilized powder containing sugars and EDTA as stabilizers

基本信息

酶学委员会(EC)编号3.5.3.3 ( BRENDA | IUBMB )
EC 号253-471-1
MDL编号MFCD00130875
NACRESNA.54

产品性质

质量水平200
生物来源bacterial (Actinobacillus spp.)
形式lyophilized powder
specific activity≥4 units/mg solid
分子量 ~100 kDa
储存温度−20℃

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, type N95 (US)

Sigma-Aldrich

推荐产品
| 首页 | 联系我们 | 会员服务 | 广告服务 | 友情链接 |
版权所有 CopyRight © 2008-2024 粤ICP备08119708号