产品说明
细胞系来源
Mink lung
细胞系描述
Derived from trypsinised lungs of several foetuses of Aleutian mink. Used for focus forming assays for murine and feline sarcoma viruses.
应用
Virus focus forming assays: MSV, feline sarcoma virus
培养基
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37℃.
其他说明
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产品性质
生物来源 | mink lung |
生长模式 | Adherent |
核型 | 2n = 30; diploid and pseudodiploid |
形态学 | Epithelial |
产品 | Not specified |
受体 | Not specified |
technique(s) | cell culture | mammalian: suitable |
运输 | dry ice |
储存温度 | −196℃ |