RCE

产品说明

细胞系来源

Rabbit corneal epithelial cells, SV40 transformed

细胞系描述

The RCE cell line, also known as K′RCE, was established after infection of primary cultured rabbit (New Zealand albino) corneal epithelial cells with a recombinant SV40 vector. The cells exhibit a cobblestone-like appearance and stratification, including desmosome formation and microvilli development. Expression of keratin AE1 was always detectable whereas AE5 was expressed only at confluency.

应用

Corneal epithelial research

培养基

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 5μg/ml Insulin + 10ng/ml EGF + 0.1μg/ml Cholera toxin + 0.5% Dimethyl Sulphoxide (DMSO) + 15% Foetal Bovine Serum (FBS) (Heat Inactivated).

传代培养常规

Split sub-confluent cultures (70-80%) 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37℃.

其他说明

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产品性质