产品说明
细胞系来源
Human Prostate Cancer Metastasis
细胞系描述
This Vertebral-Cancer of the Prostate (VCaP) cell line was established from prostate cancer tissue harvested from a metastatic lesion to a lumbar vertebral body of a patient with hormone refractory prostate cancer. The cells are reported to express large quantities of prostate specific antigen (PSA). This cell line also expresses prostatic acid phosphatase (PAP), cytokeratin-18 and the androgen receptor, and is androgen sensitive in vitro and in vivo. This cell line offers a cell-based model system of human prostate cancer. The cells are tumourigenic in SCID mice.
DNA图谱分析
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,12
D16S539: 9
D5S818: 12
D7S820: 9,12
THO1: 9.3
TPOX: 8,11
vWA: 18,19
培养基
DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37℃. Pellet cells by centrifugation and remove trypsin after inactivation of trypsin at each subculture.
其他说明
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产品性质
| 生物来源 | human prostate (metastasis) |
| 生长模式 | Adherent |
| 核型 | Refer to reference (Korenchuk et al., 2001) |
| 形态学 | Epithelial |
| 产品 | Not specified |
| 受体 | Androgen receptor |
| technique(s) | cell culture | mammalian: suitable |
| 相关疾病 | metastasis |
| 运输 | dry ice |
| 储存温度 | −196℃ |
