位置:首页 > 品牌 > Sigma-Aldrich >

Hybridoma Subisotyping Kit, Mouse

品牌
Sigma-Aldrich
货号
386445
包装型号
规格纯度
参考价格
6861.94 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Useful to confirm the purity of cell lines in monoclonal antibody development assays. Sufficient to perform 900 assays (150 clones).
The Hybridoma Subisotyping Kit is intended for the detection and identification of mouse monoclonal antibody subclasses IgG1, IgG2a, IgG2b, IgM and IgA in supernatants. It is also used for confirmation of monoclonality of cloned hybridoma cultures. This double antibody detection system is a highly sensitive enzyme immunoassay method incorporating specific primary antisera. Assay sensitivity is less than 1 µg/ml and can be reliably used with diluted culture supernatants. The following protocol utilizes 50 µl X 7 (350 µl) of each supernatant tested (a 1:2 dilution occurs as each sample is added to diluent in each well). When supernatant medium is precious, the medium from high producer cells can be diluted much more (1:10 or greater), with appropriate modifications in the Application of Supernatants section. When used as directed, the reagents in this kit will be sufficient for 900 determinations or 150 clones

包装

1 kit in Glass bottle

组分

Antibodies to IgA, IgG₁, IgG2a, IgG2b, IgG₃, IgM, anti-mouse peroxidase conjugate, TMB substrate solution, PBS concentrate, surfactant, and a user protocol.

警告

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

储存及稳定性

Upon arrival store the entire contents of the kit at 4°C.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

manufacturer/tradenameCalbiochem®
质量水平100
储存条件OK to freeze
储存温度2-8℃

Sigma-Aldrich

推荐产品
| 首页 | 联系我们 | 会员服务 | 广告服务 | 友情链接 |
版权所有 CopyRight © 2008-2024 粤ICP备08119708号