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C3H/10T1/2, Clone 8小鼠纤维原细胞
产品介绍
目录号
Serial
SCSP-506
标识符
Identifier
CSTR:19375.09.3101MOUSCSP506
描述
Description
这株细胞由C. Reznikoff、D. Brankow和C. Heidelberger于1972年从C3H系小鼠胚胎细胞中分离而来。建系人建议在P15前使用。该细胞为接触抑制型,在同源小鼠中不产生肿瘤,无自然转化的背景,也不含有明显的内源性转化鼠类白血病或肉瘤病毒。该细胞易于转染,容易被化学试剂转化。为了保持细胞原有的特性,应严格控制该细胞的接种密度、培养和传代周期。血清批次间的差异有可能影响细胞形态和细胞转染等实验结果。据报道,鼠痘病毒(ectromelia virus,ECTV)检测结果为阴性。本库冻存时为P10。
细胞质控结果:
1.支原体检测结果:每个批次均通过本库支原体检测,结果为阴性。
2.STR鉴定结果:
①该株细胞DNA进行小鼠细胞STR分型结果显示,扩增后图谱清晰,分型结果良好:1-1:10;1-2:16;2-1:9;3-2:14;4-2:20.3;5-5:15;6-4:18;6-7:12;7-1:26;8-1:15;11-2:16;12-1:16;13-1:17.1;15-3:25.3;17-2:14;18-3:16;19-2:12;X-1:26。
②该株细胞确为小鼠细胞,没有人源细胞污染。
动物种别
Organism
小鼠,C3H品系
性别
Gender
组织来源
Tissue and Cell Type
胚胎
形态
Morphology
成纤维细胞,贴壁生长
培养基和添加剂
Complete Growth Medium and Culture Conditions
MEM (Invitrogen, 11090081)  87 ml
FBS (Gibco)   10 ml
Glutamax(invitrogen 35050061)  1 ml
SODIUM PYRUVATE SOL(invitrogen 11360070)  1 ml
Non-essential Amino Acids, 100×(Invitrogen, 11140050)  1 ml
气相:空气,95%;二氧化碳,5%。温度:37摄氏度。
参考传代周期:必须在细胞交汇前传代
参考传代比例:2000个细胞/平方厘米
参考换液频率:如有需要,可在两次传代之间换一次液
冻存液配方:完全培养液95%,DMSO 5%
供应限制
Permits and Restrictions
A。仅供研究之用。
REFERENCEReznikoff CA, et al. Quantitative and qualitative studies of chemical transformation of cloned C3H mouse embryo cells sensitive to postconfluence inhibition of cell division. Cancer Res. 33: 3239-3249, 1973. PubMed: 4796800Terzaghi M, Little JB. Repair of potentially lethal radiation damage in mammalian cells is associated with enhancement of malignant transformation. Nature 253: 548-549, 1975. PubMed: 1167940Mondal S, Heidelberger C. Transformation of C3H/10T1/2 CL8 mouse embryo fibroblasts by ultraviolet irradiation and a phorbol ester. Nature 260: 710-711, 1976. PubMed: 1264242Smith GJ, et al. Clonal analysis of the expression of multiple transformation phenotypes and tumorigenicity by morphologically transformed 10T1/2 cells. Cancer Res. 53: 500-508, 1993. PubMed: 8425183Rapp UR, et al. Endogenous oncornaviruses in chemically induced transformation. I. Transformation independent of virus production. Virology 65: 392-409, 1975. PubMed: 165619Reznikoff CA, et al. Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division. Cancer Res. 33: 3231-3238, 1973. PubMed: 4357355Jain MK, et al. Molecular cloning and characterization of SmLIM, a developmentally regulated LIM protein preferentially expressed in aortic smooth muscle cells. J. Biol. Chem. 271: 10194-10199, 1996. PubMed: 8626582
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