EDJ#22小鼠胚胎干细胞

产品介绍

目录号 Serial	SCSP-220
标识符 Identifier	CSTR:19375.09.3101MOUSCSP220
描述 Description	小鼠全能性胚胎干细胞，每支细胞量1×10⁶。每个批次均通过本库支原体检测，结果为阴性。培养时需使用CF-1 MEF(SCSP-105R)作为饲养层细胞。
动物种别 Organism	小鼠，129S5/SvEvTac品系
性别 Gender	雄
组织来源 Tissue and Cell Type	小鼠胚胎内细胞团
形态 Morphology	球形克隆
培养基和添加剂 Complete Growth Medium and Culture Conditions	mES完全培养液配方(600 ml)： DMEM (Invitrogen 12430) 497 ml ES级FBS (biochrom S4115) 90 ml Glutamax (Invitrogen 35050) 6 ml NEAA (Invitrogen 11140) 6 ml LIF (Millipore ESG1107) 60 μl(终浓度为1000U/ml) β-Mer (Invitrogen 21985) 1.5 ml 气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
供应限制 Permits and Restrictions	A。仅供研究之用。
REFERENCE	Festing MF, et al. Revised nomenclature for strain 129 mice. Mamm. Genome. 10(8):836, 1999. PubMed: 10430671Simpson EM, et al. Genetic variation among 129 substrains and its importance for targeted mutagenesis in mice. Nat. Genet. 16(1):19-27, 1997. PubMed: 9140391Threadgill DW, et al. Genealogy of the 129 inbred strains: 129/SvJ is a contaminated inbred strain. Mamm. Genome. 8(6):390-393 1997. PubMed: 9166580Auerbach W, et al. Establishment and chimera analysis of 129/SvEv- and C57BL/6-derived mouse embryonic stem cell lines. BioTechniques 29: 1024-1028, 1030, 1032, 2000. PubMed: 11084865

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