ES-D3 [D3]小鼠胚胎干细胞

产品介绍

目录号 Serial	SCSP-202
标识符 Identifier	CSTR:19375.09.3101MOUSCSP202
描述 Description	在没有饲养层或条件培养基的情况下，ES-D3 [D3]细胞会自发分化为胚胎结构。可以将它们注射回囊胚并能进入生殖系。未分化的D3细胞可以通过基因靶向技术进行遗传修饰。每个批次均通过本库支原体检测，结果为阴性。培养时需使用昆明白MEF(SCSP-101R)作为饲养层细胞。冻存管细胞为P12。
动物种别 Organism	小鼠，129/Sv+c/+p品系
性别 Gender
组织来源 Tissue and Cell Type	小鼠囊胚内细胞团
形态 Morphology	球形克隆，贴壁生长
培养基和添加剂 Complete Growth Medium and Culture Conditions	mES完全培养液配方(600 ml)： DMEM (Invitrogen 12430) 497 ml ES级FBS (Gibco) 90 ml Glutamax (Invitrogen 35050-061) 6 ml NEAA (Invitrogen 11140-050) 6 ml LIF (Millipore ESG1107) 60 μl(终浓度为1000U/ml) β-Mer (Invitrogen 21985) 1.5 ml 气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
供应限制 Permits and Restrictions	A。仅供研究之用。
REFERENCE	Doetschman TC, et al. The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium. J. Embryol. Exp. Morphol. 87: 27-45, 1985. PubMed: 3897439Williams RL, et al. Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells. Nature 336: 684-687, 1988. PubMed: 3143916Gossler A, et al. Transgenesis by means of blastocyst-derived embryonic stem cell lines. Proc. Natl. Acad. Sci. USA 83: 9065-9069, 1986. PubMed: 3024164Doetschman T, et al. Targeted mutation of the Hprt gene in mouse embryonic stem cells. Proc. Natl. Acad. Sci. USA 85: 8583-8587, 1988. PubMed: 3186749Chambon P, et al. Genetically engineered mice containing alterations in the genes encoding retinoic acid receptor proteins. US Patent 6,486,381 dated Nov 26 2002

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