C57BL/6小鼠胚胎干细胞

产品介绍

目录号 Serial	SCSP-218
标识符 Identifier	CSTR:19375.09.3101MOUSCSP218
描述 Description	C57BL/6植入c2J囊胚时，毛色嵌合体的得率高；而植入FVB囊胚产生的嵌合体数量少。ES细胞可以进入FVB/NJ(FVB)和同源突变品系C57BL/6-Tyrc-2J(c2J)这两个供体宿主囊胚的生殖系。每个批次均通过本库支原体检测，结果为阴性。培养时需使用CF-1 MEF(SCSP-105R)作为饲养层细胞。冻存时为P26
动物种别 Organism	小鼠，C57BL/6品系
性别 Gender
组织来源 Tissue and Cell Type	小鼠胚胎内细胞团
形态 Morphology	球形克隆，贴壁生长
培养基和添加剂 Complete Growth Medium and Culture Conditions	mES完全培养液配方(600 ml)： DMEM (Invitrogen 12430) 497 ml ES级FBS (biochrom S4115) 90 ml Glutamax (Invitrogen 35050) 6 ml NEAA (Invitrogen 11140) 6 ml LIF (Millipore ESG1107) 60 μl(终浓度为1000U/ml) β-Mer (Invitrogen 21985) 1.5 ml 气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
供应限制 Permits and Restrictions	A。仅供研究之用。
REFERENCE	Brook FA, et al. The derivation of highly germline-competent embryonic stem cells containing NOD-derived genome. Diabetes. 52:205-208, 2003. PubMed: 12502514Brook FA, Gardner RL. The origin and efficient derivation of embryonic stem cells in the mouse. Proc. Natl. Acad. Sci. USA. 94: 5709-5712, 1997. PubMed: 9159137 Schuster-Gossler K, et al. Use of coisogenic host blastocysts for efficient establishment of germline chimeras with C57BL/6J ES cell lines. BioTechniques 31: 1022-1024, 1026, 2001. PubMed: 11730008 Auerbach W, et al. Establishment and chimera analysis of 129/SvEv- and C57BL/6-derived mouse embryonic stem cell lines. BioTechniques 29: 1024-1028, 1030, 1032, 2000. PubMed: 11084865 Matise M, et al., Production of targeted embryonic stem cell clones. In: Matise M, et al., Gene Targeting: A Practical Approach.Oxford, Oxford University Press, 101-132, 1999Lemckert FA, et al. Gene targeting in C57BL/6 ES cells. Successful germ line transmission using recipient BALB/c blastocysts developmentally matured in vitro. Nucleic Acids Res. 25: 917-918, 1997. PubMed: 9016649 Ledermann B, Burki K. Establishment of a germ-line competent C57BL/6 embryonic stem cell line. Exp. Cell Res. 197: 254-258, 1991. PubMed: 1959560

广州伟伯科技有限公司
手机: 13533759269
联系人: 林生