C2C12小鼠成肌细胞

产品介绍

目录号 Serial	GNM26
标识符 Identifier	CSTR:19375.09.3101MOUGNM26
描述 Description	这是D. Yaffe 和 O. Saxel建立的小鼠成肌细胞株的亚克隆(由H. Blau等人构建)。C2C12细胞株分化很快，形成可伸缩的肌管并生成特征性的肌蛋白。用骨形成蛋白2(BMP-2)处理，导致分化途径从成肌细胞转换成成骨细胞。检测表明肢骨发育畸形病毒(鼠痘)阴性。在本库通过支原体检测。
动物种别 Organism	小鼠
性别 Gender	***
组织来源 Tissue and Cell Type	组织：肌肉品系： C3H细胞类型：成肌细胞。
形态 Morphology	成肌细胞
培养基和添加剂 Complete Growth Medium and Culture Conditions	DMEM培养基(GIBCO by life technologies ,货号C11995500BT)，90%；优质胎牛血清，10%。气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
供应限制 Permits and Restrictions	A。仅供研究之用。
REFERENCE	Yaffe D, Saxel O. Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle. Nature 270: 725-727, 1977. PubMed: 563524Blau HM, et al. Plasticity of the differentiated state. Science 230: 758-766, 1985. PubMed: 2414846Katagiri T, et al. Bone morphogenetic protein-2 converts the differentiation pathway of C2C12 myoblasts into the osteoblast lineage [published erratum appears in J Cell Biol 1995 Feb;128(4):following 713]. J. Cell Biol. 127: 1755-1766, 1994. PubMed: 7798324Chow YH, et al. Improvement of hepatitis B virus DNA vaccines by plasmids coexpressing hepatitis B surface antigen and interleukin-2. J. Virol. 71: 169-178, 1997. PubMed: 8985336Kessler PD, et al. Gene delivery to skeletal muscle results in sustanined expression and systemic delivery of a therapeutic protein. Proc. Natl. Acad. Sci. USA 93: 14082-14087, 1996. PubMed: 8943064Hsu DK, et al. Identification of a murine TEF-1-related gene expressed after mitogenic stimulation of quiescent fibroblasts and during myogenic differentiation. J. Biol. Chem. 271: 13786-13795, 1996. PubMed: 8662936

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