3T3-L1小鼠胚胎成纤维细胞
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目录号 Serial | GNM25 | 标识符 Identifier | CSTR:19375.09.3101MOUGNM25 | 描述 Description | L1是通过克隆分离得到的3T3 (swiss小白鼠)的连续亚株。 当细胞从快速分裂到长满且接触抑制时,该细胞经过前脂肪向脂肪样逆转。 培液中,高血清含量可以促进脂肪积累。 在本库通过支原体检测。 | 动物种别 Organism | 小鼠 | 性别 Gender | 未知 | 组织来源 Tissue and Cell Type | 胚胎 | 形态 Morphology | 成纤维细胞 | 培养基和添加剂 Complete Growth Medium and Culture Conditions | DMEM培养基(GIBCO by life technologies ,货号C11995500BT),90%;优质胎牛血清,10%。 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。 | 供应限制 Permits and Restrictions | A。仅供研究之用。 | REFERENCE | 886: Green H, Meuth M. An established pre-adipose cell line and its differentiation in culture. Cell 3: 127-133, 1974. PubMed: 44260903491: Green H. Triglyceride-accumulating clonal cell line. US Patent 4,003,789 dated Jan 18 197732373: Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 870926032455: Scherer PE, et al. Identification, sequence, and expression of caveolin-2 defines a caveolin gene family. Proc. Natl. Acad. Sci. USA 93: 131-135, 1996. PubMed: 855259032787: Kallen CB, Lazar MA. Antidiabetic thiazolidinediones inhibit leptin (ob) gene expression in 3T3-L1 adipocytes. Proc. Natl. Acad. Sci. USA 93: 5793-5796, 1996. PubMed: 8650171 |
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