AB2.2小鼠胚胎干细胞

产品介绍

目录号 Serial	SCSP-217
标识符 Identifier	CSTR:19375.09.3101MOUSCSP217
描述 Description	该细胞系已广泛用于创建基因敲除小鼠。HPRT负突变使该品系可用于染色体工程。已经表明该小鼠ES细胞系具有生殖能力。每个批次均通过本库支原体检测，结果为阴性。我库冻存时为P24代。培养时需使用CF-1 MEF(SCSP-105R)作为饲养层细胞。
动物种别 Organism	小鼠，129S5/SvEvBrd(原129/SvEvBrd)品系
性别 Gender	雄性
组织来源 Tissue and Cell Type	小鼠胚胎内细胞团
形态 Morphology	球形克隆
培养基和添加剂 Complete Growth Medium and Culture Conditions	mES完全培养液配方(600 ml)： DMEM (Invitrogen 12430) 497 ml ES级FBS (biochrom S4115) 90 ml Glutamax (Invitrogen 35050) 6 ml NEAA (Invitrogen 11140) 6 ml LIF (Millipore ESG1107) 60 μl(终浓度为1000U/ml) β-Mer (Invitrogen 21985) 1.5 ml 气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
供应限制 Permits and Restrictions	A。仅供研究之用。
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