AE-1杂交瘤细胞抗AChE

产品介绍

目录号 Serial	HYB54
标识符 Identifier	CSTR:19375.09.3101ETCHYB54
描述 Description	动物用纯化的人红细胞乙酰胆碱脂酶免疫。脾细胞与gp2/O-Agl4骨髓瘤细胞融合。产生的抗体与人及猴的乙酰胆碱脂酶结合，但反应位点与AE-2的抗原决定簇不同。检测发现短肢畸形(鼠痘)病毒阴性。在本库通过支原体检测。
动物种别 Organism	小鼠B细胞x小鼠淋巴瘤
性别 Gender	***
组织来源 Tissue and Cell Type	B淋巴细胞,淋巴瘤
形态 Morphology	淋巴母细胞
培养基和添加剂 Complete Growth Medium and Culture Conditions	DMEM培养基(GIBCO by life technologies ,货号C11995500BT)，90%；优质胎牛血清，10%。气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
供应限制 Permits and Restrictions	A。仅供研究之用。
REFERENCE	Fambrough DM, Engel AG, Rosenberry TL. Acetylcholinesterase of human erythrocytes and neuromuscular junctions: homologies revealed by monoclonal antibodies. Proc Natl. Acad. Sci. USA 79:1078-1082, 1982. PubMed: 6175961 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2～d edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Centers for Disease Control (1993), Biosafi～ty in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C.

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