4T1小鼠乳腺癌细胞

产品介绍

目录号 Serial	TCM32
标识符 Identifier	CSTR:19375.09.3101MOUTCM32
描述 Description	4T1 是从410.4瘤株中未经诱变筛得的6-硫鸟嘌噙抗性细胞株。当注射到BALB/c 小鼠中时，4T1自发产生高转移肿瘤，可转移到肺，肝，淋巴结和大脑，同时在注射部位形成始发灶。诱导转移时不需要摘除始发灶。 4T1细胞在BALB/c小鼠中的生长与转移特性与人体中的乳腺癌十分相近。这种肿瘤是人VI期乳腺癌的动物模型。 4T1-诱导的肿瘤在手术后及未手术情况下转移的动力学相近，可以用作手术后及未手术模型。跟其他肿瘤模型相比，由于4T1的抗6-硫鸟嘌噙特性，微小的转移细胞团(少到仅仅1个)也可以在许多远端器官中检测到。没必要数淋巴结或称重器官。在本库通过支原体检测。
动物种别 Organism	小鼠
性别 Gender	***
组织来源 Tissue and Cell Type	乳腺癌
形态 Morphology	上皮细胞
培养基和添加剂 Complete Growth Medium and Culture Conditions	RPMI 1640培养基(GIBCO by life technologies ,货号C11875500BT)，90%；优质胎牛血清，10%。气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
供应限制 Permits and Restrictions	A。仅供研究之用。
REFERENCE	Pulaski BA , et al. Immunotherapy with vaccines combining MHC class II/CD80+tumor cells with interleukin-12 reduces established metastatic disease and stimulatesimmune effectors and monokine induced by interferon gamma. Cancer Immunol.Immunother. 49: 34-45, 2000. PubMed: 10782864Pulaski BA , Ostrand-Rosenberg S . Reduction of established spontaneous mammarycarcinoma metastases following immunotherapy with major histocompatibility complex classII and B7.1 cell-based tumor vaccines. Cancer Res. 58: 1486-1493, 1998. PubMed: 9537252Pulaski BA , et al. Cooperativity of Staphylococcal aureus enterotoxin B superantigen,major histocompatibility complex class II, and CD80 for immunotherapy of advancedspontaneous metastases in a clinically relevant postoperative mouse breast cancer model.Cancer Res. 60: 2710-2715, 2000. PubMed: 10825145Aslakson CJ , Miller FR . Selective events in the metastatic process defined by analysis of the sequential dissemination of subpopulations of a mouse mammary tumor.Cancer Res. 52: 1399-1405, 1992. PubMed: 1540948

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