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Anti-Synapsin-1 Antibody, clone MEGS3-10A

品牌
Sigma-Aldrich
货号
MABN1847
规格纯度
clone MEGS3-10A, from mouse
参考价格
7365.52 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Synapsin-1 (UniProt O88935; also known as Synapsin I) is encoded by the Syn1 (also known as Syn-1) gene (Gene ID 20964) in murine species. The synapins constitute a family of abundant neuronal phosphoproteins that regulate SV trafficking and neurotransmitter release at the pre-synaptic terminal. Three genes exisit in mammals, encoding altogether 11 synapsin members by alternative splicings (Synapsin Ia & Ib by SYN1, Synapsin IIa & IIb by SYN2, Synapsin IIIa through IIIf by SYN3), Syn III is the most precociously expressed isoform that has a role in the early phases of neural development and is downregulated in mature neurons. On the other hand, Syn I and Syn II are expressed at low levels at birth and their expressions progressively increase during synaptogenesis to reach a stable plateau at 1–2 months of life. The NH2-terminal region is divided in A, B, and C domains and is highly conserved among synapsin isoforms. Synapsins are regulated by postransloational phosphorylations. Domain A contains PKA and CaMKI/IV phosphorylation sites, domain B contains MAPK/Erk phosphorylation sites, and domain C is phosphorylated by Src. The c-terminal regions contain spliced domains and diverge among synapsin isoforms (domain D in Syn Ia and Ib, domain G in Syn IIa and IIb, domain H in Syn IIa, and domain J in Syn IIIa), although they all bear proline-rich regions binding to several SH3-containing proteins and additional phosphorylation sites for CaMKII, MAPK/Erk, and cdk1/5.

免疫原

Recombinant full-length mouse synapsin-1.

应用

Research Category
Neuroscience
Immunocytochemistry Analysis: The ascites prior to antibody purification detected Synapsin-1 in primary mouse cortical neurons (Courtesy of Professor Michael E. Greenberg, Harvard Medical School, Boston, MA).
Western Blotting Analysis: An 1:1000 dilution of the ascites prior to antibody purification detected a higher Synapsin-1 expression in brain lysate from a 60-day old (P60) mouse than from a 6-day old (P6) mouse (Courtesy of Professor Michael E. Greenberg, Harvard Medical School, Boston, MA).
Research Sub Category
Neurotransmitters & Receptors

质量

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Synapsin-1 in 10 µg of mouse brain tissue lysate.

目标描述

~75 kDa observed.

外形

Purified by Ion-Exchange Chromatography
Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702

产品性质

质量水平100
生物来源mouse
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆MEGS3-10A, monoclonal
species reactivitymouse
technique(s)immunocytochemistry: suitable
western blot: suitable
同位素/亚型IgG2bκ
NCBI登记号NP_038708.3
UniProt登记号O88935
运输wet ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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