产品说明
一般描述
Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCH-L1), a thiol protease belonging to the peptidase C12 family, is involved in the processing of ubiquitin precursors and of ubiquitinated proteins. UCH-L1 recognizes and hydrolyzes peptide bonds at the C-termial glycine of ubiquitin. UCH-L1 is expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Defects in UCH-L1 are the cause of Parkinson disease type 5.
免疫原
His-tagged recombinant protein corresponding to human UCHL1.
应用
Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected UCHL1 in 10 µg of human brain tissue lysate.
Immunofluorescence Analysis: A 1:2,000 dilution from a representative lot detected UCHL1 in rat brain spinal cord tissue (Gerry Shaw. EnCor Biotechnology, Inc., Gainesville, Florida).
Detect UCH-L1 using this mouse monoclonal antibody, Anti-UCHL1 Antibody, clone MCA-BH7 validated for use in western blotting & Immunofluorescence.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
质量
Evaluated by Western Blotting in rat brain spinal cord tissue lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected UCHL1 in 10 µg of rat brain tissue lysate.
目标描述
~25 kDa observed
外形
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.
Protein G Purified
储存及稳定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析说明
Control
Rat brain spinal cord tissue lysate.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | MCA-BH7, monoclonal |
| species reactivity | bovine, rat, human |
| technique(s) | immunofluorescence: suitable western blot: suitable |
| 同位素/亚型 | IgG1κ |
| NCBI登记号 | NP_004172 |
| UniProt登记号 | P09936 |
| 运输 | wet ice |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
