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Anti-Thiazide-Sensitive NaCl Cotransporter Antibody

品牌
Sigma-Aldrich
货号
AB3553
规格纯度
Chemicon®, from rabbit
参考价格
7029.8 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

特异性

Recognizes the renal thiazide-sensitive NaCl cotransporter (NCC). The antibody stains the apical plasma membrane and subapical membrane vesicles in renal distal convoluted tubule cells.

免疫原

Synthetic peptide from the N-terminal of human/rat/mouse NCC.

应用

Immunoblotting: 1:2,000 using rat kidney extract. The antibody reacts with the 160-190 kDa protein (the molecular weight varies depending on conditions for protein extraction/electrophoresis). The suggested dilution and blocking buffer is 150 mM NaCl, 10 mM Tris, pH 7.4 containing 5% low fat milk and 0.04% Tween. Suggested transfer membrane is PVDF or Nylon. Suggested gel percentage is 7%. Overnight incubation with the antibody at 2-8°C is recommended.

Immunohistochemistry: 1:500-1:2,000 on rat or mouse kidney tissue. Suggested fixative is 3% paraformaldehyde for 5 minutes by vascular perfusion. Suggested blocking buffer is PBS containing 10% normal goat serum. Suggested dilution buffer is PBS containing 1% BSA. Overnight incubation with the antibody at 2-8°C is recommended.Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
This Anti-Thiazide-Sensitive NaCl Cotransporter Antibody is validated for use in IH, WB for the detection of Thiazide-Sensitive NaCl Cotransporter.

目标描述

160-190 kDa

联系

Replaces: AB3564P

外形

Affinity Purified immunoglobulin in PBS containing no preservatives.
ImmunoAffinity Purified

储存及稳定性

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

分析说明

Control
POSITIVE CONTROL: Rat kidney.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式affinity purified immunoglobulin
antibody product typeprimary antibodies
克隆polyclonal
纯化方式affinity chromatography
species reactivityrat, rabbit, mouse, human
manufacturer/tradenameChemicon®
technique(s)immunohistochemistry: suitable
western blot: suitable
NCBI登记号NM_000339.1
UniProt登记号P55017
运输wet ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

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