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Anti-Sortilin Antibody, clone F11

品牌
Sigma-Aldrich
货号
MABN1792
规格纯度
clone F11, from mouse
参考价格
6190.47 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Sortilin (UniProt Q99523; also known as 100 kDa NT receptor, Glycoprotein 95, Gp95, Neurotensin receptor 3, NT3, NTR3) is encoded by the SORT1 (also known as LDLCQ6, NT3) gene (Gene ID 6272) in human. Sortlin belongs to the family of sorting receptors that are characterized by the Vps10p domain, which creates a tunnel cavity with its ten-bladed β-propeller for the binding of soluble ligands. These family members also contain a short cytoplasmic tail that harbors recognition motifs for subcellular sorting adaptor proteins. Sortilin is reported to bind PCSK9 in the trans-Golgi network with high-affinity in HepG2 cells. Sort1 knockout mice exhibit low levels of PCSK9 in plasma and its subcellular localization in hepatocytes is also altered. Sortilin is also found to co-localize with amyloid precursor protein (APP) in neurites and it facilitates APP processing toward production of sAPPα, possibly by optimizing APP as substrate for α-secretase cleavage. Sortilin is initially produced with a signal (a.a. 1-33) and a propeptide (a.a. 34-77) sequence, the removal of which yields the mature receptor (a.a. 78-831) with a large luminal/extracellular (a.a. 78-555) portion, a transmembrane region(a.a. 756-778), and a short cytoplasmic tail (a.a.779-831). Like the other family members (MPRs and SorLA), sortilin resides both on the membrane of intracellular vesicles and on the plasma membrane. Altered sortilin expressions due to SORT1 genetic variations affect serum levels of low density lipoprotein cholesterol (LDL-C) and contribute to the LDL-C level quantitative trait locus 6 (LDLCQ6) and susceptibility to myocardial infarction.

免疫原

Epitope: Extracellular domain
Recombinant protein corresponding to the extracellular domain of human Sortilin.

应用

Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
Immunocytochemistry Analysis: A representative lot detected cellular localization of endogenous sortilin in isolated hippocampal neurons from new born (P0) wild-type, but not Sort1−/− C57BL/6J mice (Gustafsen, C., et al. (2013). J Neurosci. 33(1):64-71).
Immunocytochemistry Analysis: Representative lots detected cellular localization of exogenously expressed sortilin in transfected HEK293 cells by fluorescent immunocytochemistry (Larsen, J.V., et al. (2014). Biochem J. 457(2):277-288; Gustafsen, C., et al. (2013). J Neurosci. 33(1):64-71; Larsen, J.V., et al. (2010). Mol. Cell. Biol. 30(17):4175-4187; Nielsen, M.S., et al. (2007). Mol. Cell. Biol. 27(19):6842-6851).
ELISA Analysis: A representative lot was employed as the detection antibody in sandwich ELISA. A positive correlation was found between PCSK9 and soluble sortilin in human serum samples from healthy individuals (Gustafsen, C., et al. (2014). Cell Metab. 19(2):310-318).
Western Blotting Analysis: A representative lot detected different distribution of Sortilin than SorLA among subcellular fractions obtained by gradient centrifugation (Nielsen, M.S., et al. (2007). Mol. Cell. Biol. 27(19):6842-6851).
Anti-Sortilin Antibody, clone F11 is an antibody against Sortilin Antibody for use in Immunohistochemistry (Paraffin), Immunocytochemistry, Western Blotting and ELISA.

质量

Evaluated by Immunohistochemistry in human cerebral cortex tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Sortilin in human cerebral cortex tissue.

目标描述

~91 kDa calculated

外形

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆F11, monoclonal
species reactivitymouse, human
technique(s)ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
同位素/亚型IgG1κ
NCBI登记号NP_002950.3
UniProt登记号Q99523
运输wet ice
Gene Informationhuman ... SORT1(6272)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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