产品说明
一般描述
Protein G purified mouse monoclonal antibody. Recognizes the ~140-145 kDa Tie2 protein.
Recognizes the ~140-45 kDa Tie2 protein.
This Anti-Tie2 Mouse mAb (Ab33) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Tie2.
免疫原
Human
a recombinant protein consisting of amino acids 1-745 of human Tie2 fused to a His•Tag
包装
50 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
应用
Frozen Sections (see comments)
Immunoblotting (0.2-1 µg/ml, chemiluminescence)
Immunoprecipitation (see comments)
Paraffin Sections (not recommended)
警告
Toxicity: Standard Handling (A)
外形
In 150 mM NaCl, 100 mM Tris-glycine, 30% glycerol, pH 7.4.
重悬
Following initial thaw, aliquot and freeze (-20°C).
其他说明
Lin, P., et al. 1998. Proc. Natl. Acad. Sci. USA95, 8829.
Wong, A.L., et al. 1997. Circ. Res.81, 567.
Peters, K.G., et al. 1998. Br. J. Cancer77, 51.
For frozen sections, tissues were treated with Triton™-X-100 detergent. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | Ab33, monoclonal |
| 形式 | liquid |
| 包含 | ≤0.1% sodium azide as preservative |
| species reactivity | porcine, mouse, rat, human |
| manufacturer/tradename | Calbiochem® |
| 储存条件 | OK to freeze avoid repeated freeze/thaw cycles |
| 同位素/亚型 | IgG1 |
| 运输 | wet ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
