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Anti-Potassium Channel Kir3.4 Antibody, clone 8F4.1

品牌
Sigma-Aldrich
货号
MABS1721
规格纯度
clone 8F4.1, from mouse
参考价格
5352.52 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

G protein-activated inward rectifier potassium channel 4 (UniProt P48544; also known as Cardiac inward rectifier, CIR, Heart KATP channel, Inward rectifier K(+) channel Kir3.4, GIRK-4, IRK-4, KATP-1, Potassium channel, inwardly rectifying subfamily J member 5) is encoded by the KCNJ5 (also known as GIRK4, LQT13) gene (Gene ID 3762) in human. Inward rectifier K+ channels (Kirs) modulate cell membrane potential and are classified as K+ transport channels (Kir1.x, Kir4.x, Kir5.x, Kir7.x), classic Kir (Kir2.x), G-protein-gated Kirs (Kir2.x and Kir3.x), and ATP-sensitive Kirs (Kir6.x). Kirs mediate diverse physiological functions of both excitable and nonexcitable epithelial cells, such as cell adhesion-migration, volume regulation, apoptosis, and proliferation. While a large fraction of Kir3.4 (GIRK4; KCNJ5) subunits is processed to the cell membrane to form homomultimers, Kir3.4 subunits also assemble into heteromeric channels with Kir3.1 (GIRK1; KCNJ3) subunits in atrial myocytes or Kir3.2 (GIRK2; KCNJ6) in the cerebellum. Upon agonist activation of appropriate GPCRs, heteroterameric Kir3.4 channels are opened via interaction with subunits of the pertussis toxin-sensitive class (Gi/o) G-proteins, resulting in membrane hyperpolarization and/or reduced excitability. Whereas homotetrameric Kir3.4 channels can be activated by high intracellular Na+ independent of G-protein. Human Kir3.4 is a 419-amino acid multi-pass membrane (a.a. 87-111, 136-147, 164-185) protein that contains two small extraceullar regions (a.a. 112-135 and 155-163) and a pore-forming domain (a.a. 148-154), having both its N- and C-terminal ends exposed intracellularly (a.a. 1-86 and a.a. 186-419).

特异性

Clone 8F4.1 detects human potassium channel Kir3.4 by targeting an epitope within the C-terminal cytoplasmic domain.

免疫原

GST-tagged human potassium channel Kir3.4 C-terminal end fragment.

应用

Detect K(+) channel Kir3.4 using this mouse monoclonal Anti-Potassium Channel Kir3.4, clone 8F4.1, Cat. No. MABS1721, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
Research Category
Signaling
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected potassium channel Kir3.4 in human adrenal gland and kidney tissue sections.

质量

Evaluated by Western Blotting in human adrenal gland tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected potassium channel Kir3.4 in 10 µg of human adrenal gland tissue lysate.

目标描述

~47 kDa observed. 47.67 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified
Format: Purified
Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702

产品性质

质量水平100
生物来源mouse
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆8F4.1, monoclonal
species reactivityhuman
technique(s)immunohistochemistry: suitable (paraffin)
western blot: suitable
同位素/亚型IgG1κ
NCBI登记号NP_000881
UniProt登记号P48544
运输ambient

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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