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Anti-SCAP Antibody, clone 9D5

品牌
Sigma-Aldrich
货号
MABS1249
规格纯度
clone 9D5, from mouse
参考价格
5352.52 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Sterol regulatory element-binding protein cleavage-activating protein (UniProt P97260; also known as SCAP, SREBP cleavage-activating protein) is encoded by the SCAP gene (Gene ID 100689048) in hamster species. SCAP mediates the activation of membrane-bound transcription factors SREBPs (sterol-regulatory element binding proteins) for transcribing genes encoding cholesterol biosynthetic enzymes. In sterol-deprived cells, SCAP escorts SREBPs from ER to Golgi, where active fragments of SREBPs are released from membrane by proteolysis. Sterol accumulation inuduces a negative regulatory mechanism, where SCAP becomes bound by Insigs and trapped in the ER, preventing further delivery of SREBPs to the Golgi for proteolytic release. SCAP spans the ER membrane eight times (a.a. 19-39, 280-300, 313-333, 345-365, 402-422, 424-444, 519-539, 709-729) with
4 lumenal and 5 cytoplasmic regions, having both its N- and C-terminal ends at the cytoplasmic side (a.a. 1-18 & 730-1276). The C-terminal domain of SCAP mediates association with SREBPs, while transmembrane helices 2–6 comprise the sterol-sensing domain that mediates sterol-induced binding of SCAP to Insigs. Mutations within the sterol-sensing region disrupt Insig binding and prevent sterol-mediated ER retention of SCAP-SREBP.

特异性

Clone 9D5 detected the target band in lysate and membrane extract from CHO-K1 cells, but not from the SCAP-deficient SRD-13A CHO cell line.

免疫原

Epitope: The fourth lumenal domain.
His-tagged recombinant fragment corresponding to the fourth lumenal domain of hamster SCAP.

应用

Anti-SCAP Antibody, clone 9D5 is an antibody against SCAP for use in Western Blotting, Immunocytochemistry.
Western Blotting Analysis: 5 µg/mL from a representative lot detected SCAP in 25 µg of whole cell lysate and 50 µg of membrane extract from CHO-K1 cells, but not from the SCAP-deficient SRD-13A CHO cells (Courtesy of Linda Donnelly, Department of Molecular Genetics, UT Southwestern Medical Center, Dallas, TX, USA).
Western Blotting Analysis: A representative lot detected exogenously expressed SCAP co-immunoprecipitated with the exogenously expressed Insig-1 and Insig-2 upon 25-hydroxycholesterol (25-HC) treatment of a SCAP-deficient CHO cell line SRD-13A that had been transfected to co-express SCAP with either myc-tagged Insig-1 or Insig-2 (Lee, P.C., and DeBose-Boyd, R.A. (2010). J. Lipid Res. 51(1):192-201).
Western Blotting Analysis: Representative lots detected similar level of SCAP in the membrane extracts from CHO cells with or without SREBP processing inhibitor 25-hydroxycholesterol (25-HC) treatment regardless whether the cells′ 25-HC-resistant status (Lee, P.C., and DeBose-Boyd, R.A. (2010). J. Lipid Res. 51(1):192-201; Lee, P.C., et al. (2007). J. Lipid Res. 48(9):1944-1954).
Western Blotting Analysis: A representative lot detected the endogenous SCAP immunoprecipitated from CHO cell lysate by a polyclonal SCAP antibody (Sakai, J., et al. (1997). J. Biol. Chem. 272(32):20213-20221).
Immunocytochemistry Analysis: A representative lot localized the overexpressed hamster SCAP at ER and nuclear envelope by fluorescent immunocytochemistry staining of 3% paraformaldehyde-fixed, 0.01% saponin-permeabilized CHO stable transfectants (Sakai, J., et al. (1997). J. Biol. Chem. 272(32):20213-20221).

质量

Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be IgG2bκ.

目标描述

~150 kDa observed. Target band size appears larger than the calculated molecular weight of 139.5 kDa due to glycosylation. Uncharacterized band(s) may appear in some lysates.

外形

Protein G purified.
Format: Purified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702

产品性质

质量水平100
生物来源mouse
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆9D5, monoclonal
species reactivityhamster
technique(s)immunocytochemistry: suitable
western blot: suitable
同位素/亚型IgG2bκ
NCBI登记号NP_001230965
UniProt登记号P97260

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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