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Anti-SUMO2/3 Antibody, clone 3H12

品牌
Sigma-Aldrich
货号
MABS1139
规格纯度
clone 3H12, from rat
参考价格
4761.58 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Small ubiquitin-related modifier 2 (UniProt P61956; a.k.a. HSMT3, Sentrin-2, SMT3 homolog 2, Smt3B, SUMO-2, Ubiquitin-like protein SMT3B) and 3 (UniProt P55854; a.k.a. SMT3 homolog 1, Smt3A, SUMO-3, Ubiquitin-like protein SMT3A) are encoded by the SUMO2 (a.k.a. SMT3B, SMT3H2; Gene ID 6613) and SUMO3 (a.k.a. SMT3A, SMT3H1; Gene ID 6612) genes in human. SUMOylation, protein posttranslation modification by small ubiquitin-like modifier (SUMO), is a signaling event in many cellular processes. SUMO proteins are translated as immature precursors and subsequently converted to their mature forms through the activity of sentrin/SUMO-specific proteases (SENPs). As is the case with ubiquitination, SUMOylation is a reversible process. SUMO E1 activating enzyme, E2 conjugating enzyme, and E3 ligase mediate SUMOylation of substrate proteins, while SENPs are responsible for the deSUMOylation. SUMOylation usually occurs at lysine residues in the consensus KxD/E motif, although not all such lysines become SUMOylated and SUMOylation can also occur on lysine residues outside of this motif. SUMO2 and 3 share 97% identity at the amino acid level, while SUMO1 shares approximately 50% and SUMO4 shares about 87% identity with SUMO2/3. SUMO4 is the only SUMO member whose ctivation and conjugation has not been demonstrated. In addition to difference in their target substrates, SUMO2/3 can be SUMOylated and form chains, whereas SUMO1 cannot and may serve as chain terminator.

特异性

Clone 3H12 detects SUMO2/3 and SUMO2/3-modified (SUMOylated) proteins. Clone 3H12 reacts with SUMO2/3, but not SUMO1 (Uchimura, Y., et al. (2006). J. Biol. Chem. 281(32):23180-23190).

Predicted to react with a broad-range of mammalian species based on high sequence homology.

免疫原

GST-tagged recombinant full-length human SUMO3 (Uchimura, Y., et al. (2006). J. Biol. Chem. 281(32):23180-23190).

应用

Immunocytochemistry Analysis: Undiluted hybridoma culture supernatant from a representative lot detected SUMO2/3 immunoreactivity in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized HeLa cells.

Immunocytochemistry Analysis: 10 µg/mL of purified clone 3H12 detected SUMO2/3 immunoreactivity in primary mouse neural progenitor cells (Courtesy of Taro Tachibana, Ph.D., Osaka City University, Japan).

Western Blotting Analysis: 1 µg/mL of purified clone 3H12 detected SUMOylated proteins in HeLa cell lysate (Courtesy of Taro Tachibana, Ph.D., Osaka City University, Japan).

Immunocytochemistry Analysis: A representative lot detected SUMO-2/3 nuclear immunoreactive foci co-localized with those of MCAF1 and SUMO1 and enriched in heterochromatin proteins by indirect immunofluorescent staining of exponentially growing C-33A cells fixed with 4% paraformaldehyde and permeabilized by 0.2% Triton X-100. Trasfecting C-33A cells with SUMO2/3 shRNA or Discosoma sp. red fluorescent protein (DsRed) fusion containg SUMO2/3-interacting sequence (MCAF1 a.a. 965-975) abolished nuclear staining by clone 3H12 (Uchimura, Y., et al. (2006). J. Biol. Chem. 281(32):23180-23190).

Research Category
Signaling
Detect SUMO2/3 and SUMO2/3-modified (SUMOylated) proteins using this rat monoclonal Anti-SUMO2/3 Antibody, clone 3H12, Cat. No. MABS1139, validated for use in Immunocytochemistry and Western blotting.

质量

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this hybridoma culture supernatant detected SUMO2/3-modified (SUMOylated) proteins in 10 µg of HeLa cell lysate.

目标描述

Variable depending on the degree of SUMOylation and the size(s) of the SUMOylated protein(s). 10.61/10.52 kDa (SUMO2/SUMO3; propeptide removed) calculated.

外形

Unpurified.
Format: Unpurified
Rat IgG2a hybridoma culture supernatant with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702

产品性质

质量水平100
生物来源rat
抗体形式unpurified
antibody product typeprimary antibodies
克隆3H12, monoclonal
species reactivityrat, mouse, human
technique(s)immunocytochemistry: suitable
western blot: suitable
同位素/亚型IgG2aκ
NCBI登记号NP_008867
NP_008868
UniProt登记号P55854
P61956
运输dry ice
Gene Informationhuman ... SUMO2(6613)

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 1

Sigma-Aldrich

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