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Anti-P2X3 Receptor Antibody, pain

品牌
Sigma-Aldrich
货号
AB5895
规格纯度
serum, Chemicon®
参考价格
6526.19 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

特异性

P2X3

SPECIES REACTIVITIES:

Rat, mouse and primate. Other species have not been tested.

免疫原

Synthetic peptide corresponding to the C-terminus of rat P2X3.

应用

Detect P2X3 Receptor using this Anti-P2X3 Receptor Antibody, pain validated for use in IC, IH & WB.
Research Category
Neuroscience
Immunoblotting: 1:500-1:1,000

Immunohistochemistry: 1:500-1:1,000

Immunocytochemistry: 1:500-1:1,000

Optimal working dilutions must be determined by the end user.

APPLICATION NOTES FOR AB5895

IMMUNOHISTOCHEMISTRY

Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.

Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5895. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.

IMMUNOCYTOCHEMISTRY

P2X3 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as described above.
Research Sub Category
Neurotransmitters & Receptors

Neuroinflammation & Pain

外形

Rabbit serum. Liquid. Contains 0.05% sodium azide.

储存及稳定性

Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式serum
antibody product typeprimary antibodies
克隆polyclonal
species reactivityhuman, primate, rat
manufacturer/tradenameChemicon®
technique(s)immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI登记号NM_002559.2
UniProt登记号P56373
运输dry ice

安全信息

储存分类代码10 - Combustible liquids
WGKWGK 1

Sigma-Aldrich

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