Anti-Tbr2 Antibody

产品说明

一般描述

Eomesodermin homolog (UniProt O54839; also known as T-box brain protein 2, T-brain-2, TBR-2) is encoded by the Eomes (also known as Tbr2, C77258) gene (Gene ID 13813) in murine species. Tbr2 is a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. Trb2 has been shown to play a role in the development of the neocortex and functions in the differentiation pathway of radial glia to post-mitotic projection neuron.

特异性

Expected to react with both spliced isoforms of murine Tbr2.

免疫原

KLH-conjugated linear peptide corresponding to mouse Tbr2.
Epitope: Transcription Activation Domain

应用

This Anti-Tbr2 antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Tbr2.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Tbr2 in mouse cerebral cortex and mouse cerebellum tissue.
Western Blot (SNAP ID) Analysis: 0.5 µg/ml from a previous lot detected Tbr2 in 10 µg of E13-14 mouse brain lysate.
Immunohistochemistry Analysis: 1:100 dilution from a previous lot detected Tbr2 in mouse and human brain.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

质量

Evaluated by Western Blot in E13-14 mouse brain lysate.

Western Blot Analysis: 0.05 µg/ml of this antibody detected Tbr2 in 10 µg of E13-14 mouse brain lysate.

目标描述

~85/95 kDa observed. Target bands appear larger than the calculated molecular size of 74.8 kDa (isoform 1) and 72.7 kDa (isoform 2).

外形

Affinity purified
Purified chicken polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
E13-14 mouse brain lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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