产品说明
特异性
Recognizes NMDAR2B. By Western blot it recognizes a 180 kDa band in rat brain membranes. Also recognizes a 70 kDa band (thought to be a contaminant). No reactivity to NMDAR2A or NMDAR2C by Western blot. Immunolabeling is blocked by preadsorption of AB1557 with the C-terminal fusion protein that was used to generate the antibody.
免疫原
C-Terminal Fusion Protein of the NMDAR2B (Amino acids 984-1104).
应用
Anti-NMDAR2B Antibody, pain detects level of NMDAR2B & has been published & validated for use in IP & WB.
Western blotting 1:200-1:1,000 (NMDAR2B is present in high concentrations in the hippocampus)
Immunoprecipitation using solubilized hippocampal slices. 3 μL will (under appropriate conditions) quantitatively immunoprecipitate all NMDAR2B in 200 μg of rat brain lysate.
Not suggested for use in immunohistochemistry.
Note: Above dilutions are with 35S-protein A; with ECL dilutions may need to be considerably higher to obtain specific immunolabeling.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
目标描述
180 kDa
外形
Unpurified
Rabbit antiSerum. Liquid, no preservatives.
储存及稳定性
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
分析说明
Control
Brain tissue, Rat Purkinje cell dendrites, rat hippocampus lysate, HEK 293 cells expressing NR2B
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 抗体形式 | serum |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| species reactivity | mouse, rat, human |
| manufacturer/tradename | Chemicon® |
| technique(s) | immunoprecipitation (IP): suitable western blot: suitable |
| 适用性 | not suitable for immunohistochemistry |
| NCBI登记号 | NM_000834.2 |
| UniProt登记号 | Q13224 |
| 运输 | dry ice |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
