Anti-OSBP Antibody, clone 1A6.2

产品说明

一般描述

Oxysterol-binding protein 1 (UniProt P22059) is encoded by the OSBP (also known as OSBP1) gene (Gene ID 5007) in human. OSBP mediates the transport of cholesterol and phosphatidylinositol 4-phosphate (PtdIns4P, PI-4-P, PI4P) between endoplasmic reticulum (ER) and Golgi. OSBP links ER and trans-Golgi membranes at the ER-Golgi membrane contact sites (MCSs) and shuttles sterol into the Golgi and PI4P back to the ER, where it is hydrolyzed by the PI4P-phosphatase Sac1. OSBP contains an FFAT (phenylalanines (FF) in an acidic tract) motif (a.a. 358-364) that binds ER-resident VAP protein and an N-terminal pleckstrin homology (PH) domain (a.a. 88-181) that binds PI4P. OSBP and OSBP-related protein 4 (ORP4) are reported to be involved in virus-induced membrane alterations essential for viral replication organelle formation. OSBP knockdown inhibits virus replication, whereas overexpression of OSBP or ORP4 counteracts the antiviral effects of the OSBP/ORP4 antagonist OSW-1.

特异性

Clone 1A6.2 targets an epitope in the C-terminal region of human OSBP.

免疫原

Epitope: C-terminal region.
GST-tagged recombinant human OSBP C-terminal fragment.

应用

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected OSBP in human cerebral cortex, small intestine, and kidney tissue sections.
Research Sub Category
Vesicular Trafficking
Research Category
Signaling
This Anti-OSBP Antibody, clone 1A6.2 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of OSBP.

质量

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected OSBP in 10 µg of Jurkat cell lysate.

目标描述

~90 kDa observed. 89.42 kDa calculated. Uncharacterized band(s) may appear in some lysates.

外形

Protein G purified.
Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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