产品说明
一般描述
The POU5F1 gene expresses the POU-domain transcription factor, Octamer-4 (Oct-4), which is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state.
免疫原
Recombinant protein corresponding to human Oct-4.
应用
Research Sub Category
Transcription Factors
Anti-Oct-4 Antibody, clone 9B7 detects level of Oct-4 & has been published & validated for use in WB, IH & IF.
Research Category
Stem Cell Research
Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in human lung tissue.
Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in COS7 cells.
质量
Evaluated by Western Blot in mouse embryonic stem cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Oct-4 in 10 µg of mouse embryonic stem cell lysate.
目标描述
~39 kDa observed
外形
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
Mouse embryonic stem cell lysate
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | 9B7, monoclonal |
| species reactivity | mouse |
| species reactivity (predicted by homology) | human (immunogen homology) |
| technique(s) | immunofluorescence: suitable immunohistochemistry: suitable western blot: suitable |
| 同位素/亚型 | IgG1κ |
| NCBI登记号 | NP_002692 |
| UniProt登记号 | Q01860 |
| 运输 | wet ice |
| Gene Information | human ... POU5F1(5460) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
