产品说明
一般描述
Cyclin-dependent kinase 4 inhibitor B (UniProt P42772; also known as MTS-2, Multiple tumor suppressor 2, p14-INK4b, p15-INK4b, p15INK4B) is encoded by the CDKN2B (also known as MTS2) gene (Gene ID 1030) in human. The INK and CIP/KIP families of cyclin dependent kinase inhibitors (CDKis) protect cells from oncogenic signals-initiated cellular transformation. INK4a and INK4b (encoded by the CDKN2A and CDKN2B gene, respectively) constitute the INK4 subfamily of CDKis. INK4a and INK4b are induced by oncogenic Ras and are involved in cell cycle G1 arrest and cellular senescence. Inactivation of CDKN2A and CDKN2B are found in a wide variety of human malignancies and murine tumor models.
特异性
Clone GF10 (10C7A6), Cat. No. MABE1664 and clone GF7 (7CIG4), Cat. No. 05-430 correspond to two independent clones originated from the same mouse host. Clone GF10 (10C7A6) is recommended as an alternative to clone GF7 (7CIG4), however these two clones are expected to recognize different epitopes, exhibit different target-binding affinity and species cross-reactivity. Cross-reactivity of clone GF10 (10C7A6) toward spliced isoform 2 (p10; UniProt P42772-2) has not been confirmed.
免疫原
Recombinant full-length human INK4b (p15).
应用
Anti-INK4b (p15) Antibody, clone GF10 (10C7A6) is an antibody against INK4b (p15) for use in Western Blotting.
质量
Evaluated by Western Blotting in HEK293T cell lysate.
Western Blotting Analysis: A 1:250 dilution of this antibody detected INK4b (p15) in 50 µg of HEK293T cell lysate.
目标描述
~15 kDa observed. 14.72 kDa (isoform 1; p15) and 8.078 kDa (isoform 2; p10) calculated.
联系
Replaces: 05-430
外形
Mouse monoclonal IgG1κ hybridoma culture supernatant with 0.05% sodium azide.
Unpurified
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | culture supernatant |
| antibody product type | primary antibodies |
| 克隆 | GF10 (10C7A6), monoclonal |
| species reactivity | human |
| technique(s) | western blot: suitable |
| 同位素/亚型 | IgG1κ |
| NCBI登记号 | NP_004927 |
| UniProt登记号 | P42772 |
| Gene Information | human ... CDKN2B(1030) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
