Anti-ICAD/DFF Antibody, NT (a.a. 2-21 of mouse ICAD)

产品说明

特异性

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated Dnase. A human DNA fragmentation factor (DFF) was identified recently which was cleaved by caspase-3 during apoptosis (Liu et al. 1997). Mouse homologue of human DFF was identified as a Dnase inhibitor designated ICAD (Enari et al. 1998; Sakahira et al. 1998). Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei (Enari et al. 1998; Sakahira et al. 1998). Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which cause Dnase activation and DNA degradation, is the hallmark of apoptotic cell death (Wylile et al. 1998).

免疫原

Epitope: N-terminus (a.a. 2-21 of mouse ICAD)
Polyclonal antibody raised against a peptide corresponding to amino acids 2-21 of mouse ICAD (Enari et al. 1998).

应用

Anti-ICAD/DFF Antibody, N-terminus (a.a. 2-21 of mouse ICAD) is an antibody against ICAD/DFF for use in WB.
Research Sub Category
Apoptosis - Additional
Research Category
Apoptosis & Cancer
Western blot: 1:500-1:1000

Mouse lung tissue lysate can be used as a positive control and a 45 kDa band should be detected.

Optimal working dilutions must be determined by end user.

外形

Affinity purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide.
Format: Purified

储存及稳定性

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

产品性质

安全信息