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Anti-Brain Derived Neurotrophic Factor Antibody, pro

品牌
Sigma-Aldrich
货号
AB5613
规格纯度
serum, Chemicon®
参考价格
6780.06 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Brain-derived neurotrophic factor (UniProt P23560; also known as Abrineurin, BDNF, Neurotrophin) is encoded by the BDNF (also known as BULN2) gene (Gene ID 627) in human. BDNF is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT3, and NT4/5. BDNF mediates its neurotrophic properties by signaling through the high affinity cell surface receptor gp145/trkB. BDNF is initially produced with a signal peptide (a.a. 1-18) and a propeptide (a.a. 19-128) sequence, the removal of which yields the mature BDNF (a.a. 129-247).

特异性

This polyclonal antiserum detects prepro- and pro-BDNF, as well as prepro-/pro-BDNF-derived N-terminal fragments that contain the propeptide sequence (a.a. 19-128), but not mature BDNF or C-terminal fragments without the propeptide sequence. No cross-reactivity to other proneurotrophins.

免疫原

Epitope: Propeptide.
KLH-conjugated linear peptide corresponding to a region within human BDNF propeptide sequence.

应用

Anti-Brain Derived Neurotrophic Factor Antibody, pro is an antibody proBDNF for use in Immunohistochemistry and Western Blotting.
Research Category
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins

Neuroinflammation & Pain
Western blot: 1:1,000-1:5,000

Immunohistochemistry: 1:1,000-1:5,000

Optimal working dilutions must be determined by the end user.

目标描述

~35 kDa (glycosylated prepro-/pro-BDNF), ~50-65 kDa (glycosylated prepro-/pro-BDNF dimer), and ~26-28 kDa (non-glycosylated prepro/pro-BDNF or glycosylated prepro-/pro-BDNF N-terminal fragments) observed. Target bands appear larger than the calculated molecular weights of 27.82/28.87/29.82/37.12/31.12 kDa (human prepro-BDNF isoform 1/2/3/4/5) and 25.77/26.82/27.77/35.07/29.06 kDa (human pro-BDNF isoform 1/2/3/4/5) due to glycosylation and/or dimerization. Uncharacterized band(s) may appear in some lysates.

外形

Rabbit serum. Lyophilized. Reconstitute with 50 μL of sterile distilled water.

储存及稳定性

Maintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain at -20°C undiluted for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (ACS grade or better) can be added (1:1) for additional stability.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式serum
antibody product typeprimary antibodies
克隆polyclonal
species reactivityrat, mouse, human
manufacturer/tradenameChemicon®
technique(s)immunohistochemistry: suitable
western blot: suitable
NCBI登记号NM_001143809
NM_001143810
NM_001709
NM_170731
NM_170734
UniProt登记号P23560
运输dry ice
Gene Informationhuman ... BDNF(627)

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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